Cell Line Description:
This cell line was initiated on April 27, 1992 and took 32 months to establish. The cells are negative for expression of Her2-neu but positive for expression of p53. HCC38 was initiated from a 50-year-old white female with a prior history of leiomyosarcoma; her mother died of breast cancer. The tumor was classified as TMN Stage IIB, Grade 3, with 3/28 lymph node metastasis. HCC38 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 [EGP2] and for cytokeratin 19, and is negative for expression of estrogen receptor (ER) and progesterone receptor (PR). HCC38 contains a homozygous deletion at 3p12. DNA from an EBV- transformed lymphoblastoid cell line, HCC38 BL, from the same patient is available as ATCC CRL-2346D.
Quality Control Specifications
DNA Quality Control Information:
· > 100 kb in size by agarose gel electrophoresis
· No RNA detected in the agarose gel
· A260/A280 ratio ³ 1.8
· Tested and verified for PCR amplification and restriction digestion.