WSS-1 [WS-1] (ATCC® CRL-2029)

Organism: Homo sapiens, human  /  Cell Type:: Transformed with adenovirus 5 DNA  /  Tissue: kidney  / 

Organism Homo sapiens, human
Tissue kidney
Cell Type Transformed with adenovirus 5 DNA
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2   [Cells contain Adenovirus]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age fetus
Storage Conditions liquid nitrogen vapor phase
Derivation

This line was derived from the human embryonic kidney line, 293 (see ATCC CRL-1573). 293 cells were transfected with an expression plasmid containing cDNA encoding the rat GABA-A receptor alpha 1, beta 2 and gamma 2 subunits. Genomic integration of the transfected alpha and gamma subunit genes was confirmed; however, the beta subunit sequences were not detected in Northern and Southern blots.

Stable transfectant expressing a type I GABAA receptor (alpha1 and gamma2 subunits).

Receptor Expression
vitronectin; gamma-aminobutyric acid - A receptor (GABA-A, Type 1 BzR)
Genes Expressed
gamma-aminobutyric acid (GABA) A receptor, alpha 1 [GABRA1],gamma-aminobutyric acid (GABA) A receptor, alpha 1
Comments Produces protein gamma-aminobutyric acid (GABA) A receptor, alpha 1

Handle as potentially biohazardous material under at least Biosafety Level 2 containment.

Stable transfectant expressing a type I GABAA receptor (alpha1 and gamma2 subunits).

Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine modified to contain 4.5 g/L glucose, 1.5 g/L sodium bicarbonate and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%
Subculturing
Remove medium, and rinse the monolayer with fresh 0.25% trypsin, 0.53 mM EDTA solution. Remove the trypsin, add fresh trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Twice per week
Cryopreservation
Freeze Medium: Culture medium, 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 12
D13S317: 12,14
D16S539: 9,13
D5S818: 8
D7S820: 11,12
THO1: 7,9.3
TPOX: 11
vWA: 16,19
Name of Depositor G Wong
References

Wong G, et al. Stable expression of type I gamma-aminobutyric acidA/benzodiazepine receptors in a transfected cell line. Mol. Pharmacol. 42: 996-1003, 1992. PubMed: 1336119

Basic Documentation
References

Wong G, et al. Stable expression of type I gamma-aminobutyric acidA/benzodiazepine receptors in a transfected cell line. Mol. Pharmacol. 42: 996-1003, 1992. PubMed: 1336119