MES-SA (ATCC® CRL-1976)

Organism: Homo sapiens, human  /  Cell Type: Epithelial  /  Tissue: uterus  /  Disease: uterine sarcoma

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Organism Homo sapiens, human
Tissue uterus
Cell Type Epithelial
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease uterine sarcoma
Age 56 years
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype This is a hypo-diploid human cell line with the modal chromosome number of 45 in 48% of cells examined. The rate of polyploidy was 2.7%. There were five or six marker chromosomes present in each cell. Whereas t(5q6p), 10q+, and 14q+ markers were common in all cells examined, the chromosome combination of either double copy N21 lacking M5 (66.7%) or t(21qter--->C--->?)(M5)/single copy N21/minute metacentric (33.3%) distinguishes the two coexisting clones. Both X chromosomes were normal.
Derivation
The MES-SA cell line was established from a surgical tumor specimen obtained at the time of hysterectomy.
Initially, the cells were grown in soft agar, and later they were transferred to multiwell plates.

Clinical Data
56 years
Caucasian
female
Tumorigenic Yes
Effects
Yes, readily form colonies in soft agar.
Yes, tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.
Comments
The tumor was described as a poorly differentiated uterine sarcoma.

The nonepithelial origin of the cells was supported by ultrastructural studies and the absence of staining for mucin.

The cells are sensitive to a number of chemotherapeutic agents including doxorubicin, dactinomycin, mitomycin C, taxol and bleomycin. They are resistant to vinblastine, dacarbazine, cisplatin, melphalan, vincristine, methotrexate and etoposide.

The multiple drug resistant cell line MES-SA/Dx5 (ATCC CRL-1977) was established from MES-SA cells which were grown in the presence of doxorubicin.

Complete Growth Medium The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove spent medium, add fresh EDTA solution (0.15 g disodium EDTA, 4.0 g NaCL, 0.28 g sodium bicarbonate, 0.5 g dextrose and 0.2 g KCl dissolved in 500 mL double distilled water). Allow the cells to sit at room temperature for a few minutes, and dislodge the cells by rapping the side of the flask sharply with the palm of your hand. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Growth Conditions: Newborn calf serum may be substituted for fetal bovine serum.
STR Profile
Amelogenin: X
CSF1PO: 11
D13S317: 11,13
D16S539: 11,12
D5S818: 13
D7S820: 7,11
THO1: 6
TPOX: 8,11
vWA: 18
Population Doubling Time 22 to 24 hrs
Name of Depositor BI Sikic
Year of Origin 1980
References

Harker WG, et al. Development and characterization of a human sarcoma cell line, MES-SA, sensitive to multiple drugs. Cancer Res. 43: 4943-4950, 1983. PubMed: 6883344

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Harker WG, et al. Development and characterization of a human sarcoma cell line, MES-SA, sensitive to multiple drugs. Cancer Res. 43: 4943-4950, 1983. PubMed: 6883344