DDT1 MF-2 (ATCC® CRL-1701)

Organism: Mesocricetus auratus, hamster, Syrian golden  /  Disease: leiomyosarcoma

Organism Mesocricetus auratus, hamster, Syrian golden
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease leiomyosarcoma
Gender male
Applications
transfection host
Clinical Data
male
Receptor Expression
androgen receptor, positive; glucocorticoid; alpha 1 adrenergic; beta 2 adrenergic
Comments
Growth of DDT1 MF-2 cells is stimulated by androgens and inhibited by glucocorticoids.
Glucocorticoids inhibit the expression of the c-sis (SIS) proto-oncogene.
Beta-2-adrenergic (beta 2 adrenergic) receptor activity is functionally coupled to adenyl cyclase.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:20 is recommended
Medium Renewal: 2 to 3 times per week
To dislodge adherent cells, remove all but 5 to 10 ml of medium and vigorously shake flask. Add fresh medium, aspirate and dispense into new flasks. Subcultures may also be prepared using standard trypsinization procedures.
Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%
Name of Depositor JS Norris
References

Syms AJ, et al. Proliferation of a highly androgen-sensitive ductus deferens cell line (DDT MF-2) is regulated by glucocorticoids and modulated by growth collagen. In Vitro 19: 929-936, 1983. PubMed: 6662552

Smith RG, et al. Differential effects of androgens and glucocorticoids on regulation of androgen receptor concentrations and cell growth. J. Steroid Biochem. 20: 277-281, 1984. PubMed: 6708513

Norris JS, et al. Autocrine regulation of growth: II. Glucocorticoids inhibit transcription of c-sis oncogene-specific RNA transcripts. Biochem. Biophys. Res. Commun. 122: 124-128, 1984. PubMed: 6743325

Basic Documentation
References

Syms AJ, et al. Proliferation of a highly androgen-sensitive ductus deferens cell line (DDT MF-2) is regulated by glucocorticoids and modulated by growth collagen. In Vitro 19: 929-936, 1983. PubMed: 6662552

Smith RG, et al. Differential effects of androgens and glucocorticoids on regulation of androgen receptor concentrations and cell growth. J. Steroid Biochem. 20: 277-281, 1984. PubMed: 6708513

Norris JS, et al. Autocrine regulation of growth: II. Glucocorticoids inhibit transcription of c-sis oncogene-specific RNA transcripts. Biochem. Biophys. Res. Commun. 122: 124-128, 1984. PubMed: 6743325