N1-S1 (ATCC® CRL-1604)

Organism: Rattus norvegicus, rat  /  Tissue: liver  /  Disease: Hepatoma; Novikoff hepatoma

Organism Rattus norvegicus, rat
Tissue liver
Product Format frozen
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Hepatoma; Novikoff hepatoma
Gender male
Strain Sprague-Dawley
Storage Conditions liquid nitrogen vapor phase
The N1-S1 cell line was established from a hepatoma induced by feeding 4-dimethylaminoazobenzene to a male rat.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 4 X 104 viable cells/mL and subculture at 1 to 1.5 X 106 cells/mL (after about 3 days). Excessive clumping of cells can often be cured by trypsinizing the cells to disperse them.
Complete growth medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
Name of Depositor JE Becker