HKB-11 (CRL-12568)

Organism: Homo sapiens, human  /  Cell Type: epithelial; somatic cell hybri  /  Disease: Burkitt's lymphoma

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Organism Homo sapiens, human
Cell Type epithelial; somatic cell hybri
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2 Cells contain adenovirus and EBV viral DNA sequences

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Burkitt's lymphoma
Applications
HKB11 provides a useful system for mammalian gene expression that is easily adaptable to growth in serum protein-free medium and fermenter/ bioreactor systems.
HKB11 is a derivative of 2B8 (ATCC CRL-12569), a HAT sensitive fusion cell line derived from the P3HR-1 Burkitt's lymphoma cell line (ATCC HTB-62).
HKB11 is negative for immunoglobulin gene expression as are 293S cells.
Storage Conditions liquid nitrogen vapor phase
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Derivation
HKB11 is a derivative of 2B8 (ATCC CRL-12569), a HAT sensitive fusion cell line derived from the P3HR-1 Burkitt's lymphoma cell line (ATCC HTB-62). P3HR1 cells harbor non-immortalizing EBV. P3HR-1 cells were cloned; treated with methanesulfonic acid ethyl ester (MSE) and plated in medium containing 6-thioguanine (6TG). They were selected for deficiency in hypoxanthine-guanine phosphoribosyl transferase (HGPRT-, HPRT-). One of the clones was transfected with the neomycin resistance expression vector pSV2-neo to obtain the 2B8 cell line. A human/human hybrid cell line was made by the fusion of human embryonic kidney cells (293S) and modified Burkitt's lymphoma cells (2B8) to obtain the HKB11 cell line. HKB11 is negative for immunoglobulin gene expression as are 293S cells. HKB11 provides a useful system for mammalian gene expression that is easily adaptable to growth in serum protein-free medium and fermenter/ bioreactor systems.
Comments
HKB11 is a derivative of 2B8 (ATCC CRL-12569), a HAT sensitive fusion cell line derived from the P3HR-1 Burkitt's lymphoma cell line (ATCC HTB-62). P3HR1 cells harbor non-immortalizing EBV. P3HR-1 cells were cloned; treated with methanesulfonic acid ethyl ester (MSE) and plated in medium containing 6-thioguanine (6TG). They were selected for deficiency in hypoxanthine-guanine phosphoribosyl transferase (HGPRT-, HPRT-). One of the clones was transfected with the neomycin resistance expression vector pSV2-neo to obtain the 2B8 cell line. A human/human hybrid cell line was made by the fusion of human embryonic kidney cells (293S) and modified Burkitt's lymphoma cells (2B8) to obtain the HKB11 cell line. HKB11 is negative for immunoglobulin gene expression as are 293S cells. HKB11 provides a useful system for mammalian gene expression that is easily adaptable to growth in serum protein-free medium and fermenter/ bioreactor systems.
Complete Growth Medium The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: 1:6 to 1:10
Medium Renewal: Two to three times weekly
Cryopreservation
Freeze medium: Complete growth medium supplemented with an additional 5% fetal bovine serum and 10% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Name of Depositor Bayer Corporation
U.S. Patent Number
References

Cho MS. Human hybrid host cell for mammalian gene expression . US Patent 6,136,599 dated Oct 24 2000

Cho MS, et al. Expression system for factor VIII. US Patent 6,358,703 dated Mar 19 2002

Cho MS, et al. Establishment of a human somatic hybrid cell line for recombinant protein production. J. Biomed. Sci. 9: 631-638, 2002. PubMed: 12432229

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Cho MS. Human hybrid host cell for mammalian gene expression . US Patent 6,136,599 dated Oct 24 2000

Cho MS, et al. Expression system for factor VIII. US Patent 6,358,703 dated Mar 19 2002

Cho MS, et al. Establishment of a human somatic hybrid cell line for recombinant protein production. J. Biomed. Sci. 9: 631-638, 2002. PubMed: 12432229