L-M (ATCC® CCL-1.2)

Organism: Mus musculus, mouse  / 

Permits and Restrictions

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Organism Mus musculus, mouse
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age 100 days
Gender male
Strain C3H/An
Applications
Tested and found negative for ectromelia virus (mousepox).
Clinical Data
male
Antigen Expression
H-2k
Genes Expressed
H-2k
Virus Resistance
poliovirus 1, 2, 3
Comments
Grows in protein free medium.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium Medium 199 with Earle's BSS supplemented with 0.5% Bacto-peptone
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells from the floor of the flask, aspirate and dispense into new flasks.
Culture Conditions
Temperature: 37.0°C
Name of Depositor DJ Merchant
References

Kuchler RJ, Merchant DJ. Propagation of strain L (Earle) cells in agitated fluid suspension cultures. Proc. Soc. Exp. Biol. Med. 92: 803-806, 1956. PubMed: 13370534

Munyon WH, Merchant DJ. The relation between glucose utilization, lactic acid production and utilization and the growth cycle of L strain fibroblasts. Exp. Cell Res. 17: 490-498, 1959. PubMed: 13672205

Merchant DJ, Hellman KB. Growth of L-M strain mouse cells in a chemically defined medium. Proc. Soc. Exp. Biol. Med. 110: 194-198, 1962. PubMed: 14472756

Hsu TC, Merchant DJ. Mammalian chromosomes in vitro. XIV. Genotypic replacement in cell populations. J. Natl. Cancer Inst. 26: 1075-1083, 1961. PubMed: 13716385

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Kuchler RJ, Merchant DJ. Propagation of strain L (Earle) cells in agitated fluid suspension cultures. Proc. Soc. Exp. Biol. Med. 92: 803-806, 1956. PubMed: 13370534

Munyon WH, Merchant DJ. The relation between glucose utilization, lactic acid production and utilization and the growth cycle of L strain fibroblasts. Exp. Cell Res. 17: 490-498, 1959. PubMed: 13672205

Merchant DJ, Hellman KB. Growth of L-M strain mouse cells in a chemically defined medium. Proc. Soc. Exp. Biol. Med. 110: 194-198, 1962. PubMed: 14472756

Hsu TC, Merchant DJ. Mammalian chromosomes in vitro. XIV. Genotypic replacement in cell populations. J. Natl. Cancer Inst. 26: 1075-1083, 1961. PubMed: 13716385