pCM188 (ATCC® 87660)

Applications: YC-type (centromeric) shuttle vectorexpression vector  /  Depositors: E Herrero

Designations pCM188
Permits and Restrictions

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Depositors E Herrero
Biosafety Level 1
Vector Information
Size (kb): 8.1560001373291020
Vector: pCM188 (plasmid)
Promoters: Promoter CMV
Construct size (kb): 8.156000137329102
Features: marker(s): URA3
marker(s): ampR
operator: tetO - two repeats
other: tTA transcriptional activator
promoter: CMV
promoter: CYC1
replicon: ARS1
replicon: pMB1
MCS: BamHI...PstI
terminator: ADH1
terminator: CYC1
centromere: CEN4
YC-type (centromeric) shuttle vector
expression vector
Restriction digests of the clone give the following sizes (kb): BamHI--8.2; PstI--8.2; BamHI/XhoI--7.5, 0.52.
Yeast centromeric expression vector driven by the tetO-CYC1 promoter. One of 7 tetracycline-regulated expression vectors (ATCC 87656 - 87662) differing in the level of expression, mode of replication and selectable marker.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Gari E, et al. A set of vectors with a tetracycline-regulatable promoter system for modulated gene expression in Saccharomyces cerevisiae. Yeast 13: 837-848, 1997. PubMed: 9234672

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component of:ATCC 87663
Shipped frozen