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Classic Yeast Vectors (ATCC® 87635™)

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Application
Also useful is a version of YCp50 containing the pUC19 polylinker, YCp50-poly.
The vectors YEp24, YEp13 and YCp50 have been used for making standard, widely used S. cerevisiae libraries.
Type Strain no
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
The copy number in E. coli is moderate due to the pBR322 origin of replication, as opposed to the much higher copy number pUC origin in the pRS, pUN, and YXplac vectors.
Some yeast genes that cannot be cloned in E. coli in vectors with a pUC origin can be cloned into YEp24, YEp13 and YCp50, presumably because they are better tolerated by E. coli at the lower copy number.
Also useful is a version of YCp50 containing the pUC19 polylinker, YCp50-poly.
The vectors YEp24, YEp13 and YCp50 have been used for making standard, widely used S. cerevisiae libraries.
Product Format freeze-dried
Depositor ATCC
References

Botstein D, et al. Sterile host yeast (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17-24, 1979. PubMed: 395030

Thrash C, et al. Cloning, characterization, and sequence of the yeast DNA topoisomerase I gene. Proc. Natl. Acad. Sci. USA 82: 4374-4378, 1985. PubMed: 2989818

Broach JR, et al. Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene. Gene 8: 121-133, 1979. PubMed: 395029

Rose MD, et al. A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector. Gene 60: 237-243, 1987. PubMed: 3327750

Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863

Wang H, Stillman DJ. Transcriptional repression in Saccharomyces cerevisiae by a SIN3-lexA fusion protein. Mol. Cell. Biol. 13: 1805-1814, 1993. PubMed: 8441414

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
SDS
SDS
References

Botstein D, et al. Sterile host yeast (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17-24, 1979. PubMed: 395030

Thrash C, et al. Cloning, characterization, and sequence of the yeast DNA topoisomerase I gene. Proc. Natl. Acad. Sci. USA 82: 4374-4378, 1985. PubMed: 2989818

Broach JR, et al. Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene. Gene 8: 121-133, 1979. PubMed: 395029

Rose MD, et al. A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector. Gene 60: 237-243, 1987. PubMed: 3327750

Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863

Wang H, Stillman DJ. Transcriptional repression in Saccharomyces cerevisiae by a SIN3-lexA fusion protein. Mol. Cell. Biol. 13: 1805-1814, 1993. PubMed: 8441414