pRS413 (ATCC® 87518)

Applications: YC-type (centromeric) shuttle vector  /  Depositors: P Hieter

Permits and Restrictions

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Designations pRS413
Depositors P Hieter
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Distribution host: Escherichia coli DH5alpha
Vector Information
Size (kb): 4.97
Intact vector size: 4.970
Type of vector: phagemid
Cloning sites: ApaI XhoI SalI ClaI EcoRV EcoRI SmaI BamHI SpeI XbaI EagI
NotI SacII SacI NaeI
Polylinker sites: BssHII KpnI ApaI XhoI SalI ClaI HindIII EcoRV EcoRI PstI
SmaI BamHI SpeI XbaI EagI NotI BstXI SacII SacI BssHII
Construction: pBluescriptII KS+
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): HIS3, ->, 504-1163
replicon: f1, <-, 1473-1879
insert detection: lacZ', <-, 1880-2217
promoter for in vitro transcription: T7, ->, 2049-2068
MCS: KpnI...SacI, ->, 2076-2183
promoter for in vitro transcription: T3, <-, 2195-2214
promoter: lac, <-, 2283-2312
replicon: pMB1, 2638-2638
marker(s): ampR, <-, 3396-4256
centromere: CEN6, 4398-4514
replicon: ARSH4, 4512-4885
Vector: pRS413 (phagemid)
Promoters: Promoter for in vitro transcription T7
Construction: pBluescriptII KS+
Construct size (kb): 4.97
Features: insert detection: lacZ'
marker(s): HIS3
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: ARSH4
replicon: f1
replicon: pMB1
MCS: KpnI...SacI
centromere: CEN6
YC-type (centromeric) shuttle vector
Restriction digests of the clone give the following sizes (kb): PvuII--4.55, 0.45; BglI--2.3, 1.7, 1.0; PstI--4.05, 0.95.
A YC-type shuttle vector with HIS3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter system for visual detection of recombinants, promoters for transcription in vitro, and an extensive polylinker flanked by BssHII sites.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436

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component of:ATCC 87531
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