pRS404 (ATCC® 87515)

Applications: YI-type (integrating) shuttle vector  /  Depositors: P Hieter

Permits and Restrictions

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Designations pRS404
Depositors P Hieter
Biosafety Level 1
Vector Information
Size (kb): 4.2740001678466800
Vector: pRS404 (phagemid)
Promoters: Promoter T7
Construction: pBluescript II KS+
Marker(s):TRP1,ampR
Construct size (kb): 4.27400016784668
Features: insert detection: lacZ'
marker(s): TRP1
marker(s): ampR
promoter: T3
promoter: T7
promoter: lac
replicon: f1
replicon: pMB1
MCS: KpnI...SacI
Applications
YI-type (integrating) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--3.35, 0.95; BglI--2.6, 1.7; PvuII--3.85, 0.45.
A YI-type shuttle vector with TRP1 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter system for visual detection of recombinants, promoters for transcription in vitro, and an extensive polylinker flanked by BssHII sites.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436

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