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pGIKS-BioB

87487

Organism
Escherichia coli (Migula) Castellani and Chalmers
Classification
Enterobacteriaceae, Escherichia
Clone type
Clone
Applications
Molecular biology
Product format
Freeze-dried
Mission Collection Item
This is a Mission Collection Item.

Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit).

Characteristics

Comments
For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit).
The insert includes nt 2012 - 3052 of Genbank J04423.
The insert contains the following restriction sites (approximate kb from the 5' end): RsaI--0.33; DdeI--0.58, 0.82; SspI--1.02.
Restriction digests of the clone give the following sizes (kb): EcoRI--4.1; EcoRI/XhoI--3.0, 1.1; XhoI--4.1.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
4.1
Intact vector size
2.961
Vector name
pBluescript II KS+
Type of vector
phagemid
Host range
Escherichia coli
Vector end
XhoI; EcoRI
Cloning sites
BssHII; KpnI; ApaI; DraII; XhoI; HincII; AccI; SalI; ClaI; HindIII; EcoRI; PstI; SmaI; BamHI; XbaI; NotI; EagI; SacI
Insert detection
lacZ'
Markers
ampR
Polylinker sites
BssHII; KpnI; ApaI; DraII; XhoI; HincII; AccI; SalI; ClaI; HindIII; EcoRI; PstI; SmaI; BamHI; XbaI; NotI; EagI; SacI
Replicon
pMB1; f1

Insert information

Insert size (kb)
1.1000000000000001
Type of DNA
genomic
Insert information
DESCRIPTION OF INSERT COMPONENT:
Insert 5' end: Modification: XhoI linker
Insert 3' end: Modification: EcoRI linker
Cross references: DNA Seq. Acc.: J04423
Nucleotides 1-1041 of the insert correspond to
nucleotides 2012-3052 of J04423.
Insert end (5'): XhoI linker
Insert end (3'): EcoRI linker
Genome
Escherichia coli
Gene name
biotin synthetase
Gene product
biotin synthetase [bioB]
Gene symbol
bioB
Contains complete coding sequence
Yes

Handling information

Host
Escherichia coli DH5
Medium
Temperature
37°C
Handling notes
Restriction digests of the clone give the following sizes (kb): EcoRI--4.1;
EcoRI/XhoI--3.0, 1.1; XhoI--4.1.
- ATCC staff

The insert contains the following restriction sites (approximate kb from the 5'
end): RsaI--0.33; DdeI--0.58, 0.82; SspI--1.02.
- GenBank/EMBL/DDBJ

History

Depositors
M Follettie
Special collection
NCRR Contract
Cross references
GenBank J04423

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
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Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

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Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

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