pJL164 (ATCC® 87471)

Applications: YI-type (integrating) shuttle vectormarker deletion vector uridine monophosphate synthetase UMP synthase, orotate phosphoribosyltransferase, orotidine 5'-phosphate decarboxylase, orotate phosphoribosyltransferase 1  /  Depositors: JD Boeke

Permits and Restrictions

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Designations pJL164
Depositors JD Boeke
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host
Distribution host: Escherichia coli unknown (ATCC 33694)
Vector Information
Size (kb): 8.4
DESCRIPTION OF VECTOR:
Intact vector size: 8.400
Type of vector: phagemid
Cloning sites:
Polylinker sites:
Other unique sites: SmaI
Construction: pRS305
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): LEU2, <-
replicon: f1, <-
other: URA3 deleter cassette
replicon: pMB1
marker(s): ampR, <-
Vector: pJL164 (phagemid)
Construction: pRS305
Marker(s):LEU2,ampR
Construct size (kb): 8.4
Features: marker(s): LEU2
marker(s): ampR
other: URA3 deleter cassette
replicon: f1
replicon: pMB1
Applications
YI-type (integrating) shuttle vector
marker deletion vector uridine monophosphate synthetase UMP synthase, orotate phosphoribosyltransferase, orotidine 5'-phosphate decarboxylase, orotate phosphoribosyltransferase 1
Comments
Restriction digests of the clone give the following sizes (kb): KpnI--4.7, 4.0; XbaI--9.0; EcoRI--9.0.
ura3delta0 deletion hosts are generated by transformation with the SpeI/XhoI digested plasmid, pJL164, followed by selection on 5-FOA plates.
ura3 point mutation hosts are then differentiated from ura3delta0 deletion hosts by reversion analysis and Southern analysis.
This deleter vector is used to create designer yeast strains with a non-revertable ura3 auxotrophic marker deletion.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37°C
References

Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801

Related Products
component of:ATCC 87472
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