Size (kb): 4.2859997749328610
Vector: pBSL99 (phagemid)
Promoters: Promoter for in vitro transcription T7 (phi10)
Construct size (kb): 4.285999774932861
Features: marker(s): ampR
promoter for in vitro transcription: T7 (phi10)
MCS: MluI KpnI...SacI
MCS: SacI...KpnI MluI
coding sequence: nptII (kanR)
contains easily purifiable cassette(s) for construction aminoglycoside phosphotransferase kanamycin resistance, neomycin resistance
Restriction digests of the clone give the following sizes (kb): EcoRI--2.75, 1.4; KpnI--2.9, 1.25; MluI--2.9, 1.25; SstI--2.6, 1.5.
Plasmid useful for vector construction and generation of marked deletions and insertions in cloned DNA.
SphI and PstI sites in the nptII gene were removed by site-directed mutagenesis.
Contains a nptII (kanR) cassette flanked by multiple cloning sites duplicated in inverted orientation.
pBSL97 (ATCC 87140)
and pBSL99 (ATCC 87141)
differ in the orientations of the multiple cloning sites.