Size (kb): 7.5000000000000000
Vector: YCpIF3 (plasmid)
Promoters: Promoter for expression GAL1
Construct size (kb): 7.5
Features: marker(s): LEU2
promoter for expression: GAL1
YX-type (expression) shuttle vector
Restriction digests of the clone give the following sizes (kb): XbaI--7.5; BamHI--7.5; EcoRI--4.3, 3.5.
Care should be taken when cloning into this vector because several stop codons occur in this reading frame.
One of several S. cerevisiae shuttle expression vectors (ATCC 87078-87092) differing in selection marker, reading frame and orientation of the promoter.
Vectors YCpIF1, YCpIF2 and YCpIF3 (ATCC 87078-87080) differ only in the reading frame of the multiple cloning site.
Foreman PK, Davis RW. Cloning vectors for the synthesis of epitope-tagged, truncated and chimeric proteins in Saccharomyces cerevisiae. Gene 144: 63-68, 1994. PubMed: 7517907