Size (kb): 6.7030000686645510
Vector: pSP1 (phagemid)
Promoters: Promoter T3
Construction: pRS305 (ATCC 77140)
Construct size (kb): 6.703000068664551
Features: insert detection: lacZ'
promoter: lac, T3, T7
vector containing primer sites useful for sequencing
vector for generating nested deletions of cloned inserts
vector permitting RNA synthesis in vitro
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): KpnI--5.0, 1.8; SstI--6.7; HindIII--6.7.
Shuttle vector transforming S. pombe at high efficiency. Suitable for generating nested deletions, DNA sequencing, and producing riboprobes. Permits visual detection of recombinants by lacZalpha complementation.
Constructed from pRS305 by inserting a 1.2 kb EcoRI fragment containing ars1 into the AatII site. The NdeI site in ars1 was removed.
The order of the major features (with directions of transcription) is: < LEU2 - f1 - < lacZ' - SacI/MCS/KpnI - pMB1 ori - < ampR - ars1.
Cottarel G, et al. Two new multi-purpose multicopy Schizosaccharomyces pombe shuttle vectors, pSP1 and pSP2. Curr. Genet. 23: 547-548, 1993. PubMed: 8319314
Glycerol stock of E. coli containing the plasmid