pMB (ATCC® 77385)

Applications: expression vectorshuttle vectorvector permitting construction of fusion proteinsvector permitting visual detection of recombinants  /  Depositors: JJ McCormick

Designations pMB
Permits and Restrictions

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Depositors JJ McCormick
Biosafety Level 1
Vector Information
Size (kb): 5.5999999046325680
Vector: pMB (plasmid)
Promoters: Promoter MMTV LTR
Construction: pM (ATCC 77383)
Construct size (kb): 5.599999904632568
Features: insert detection: lacZ'
insert detection: lacZ'amb5
marker(s): ampR
promoter: MMTV LTR
replicon: pMB1
MCS: EcoRI...HindIII
MCS: NruI...HindIII
restriction site: ClaI
restriction site: NotI
restriction site: NsiI
terminator: SV40
enhancer: RSV
expression vector
shuttle vector
vector permitting construction of fusion proteins
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): PvuI--2.5, 1.8, 1.3; EcoRI--3.0, 2.6; HindIII--2.6, 1.7, 1.3; BglII--5.6; ClaI--4.3, 1.3.
The plasmid contains the following restriction sites (bp from position 0); NotI--0; EcoRI--9, 3071; ClaI--425, 1560, 1745; Drd--1398, 3583, 5452; PvuI--1596, 2953, 4741; BglII--1782; SphI--2907, 3110; NsiI--2909; HindIII--99, 1794, 3112; BsaI--4441.
Expression vector containing two lacZalpha-based multiple cloning sites, with the second containing an amber mutation and being flanked by an RSV enhancer-MMTVLTR promoter at one end and splicing and polyadenylation signals at the other.
The first site was designed for insertion of a marker selectable in mammalian cells, and the second for the sequence to be expressed.
Both sites permit detection of inserts by alpha complementation, with discrimination between the two provided by the amber mutation in the second lacZ.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Wang Q, et al. Mammalian expression vectors with modulatable promoters and two multiple cloning sites. Gene 119: 155-161, 1992. PubMed: 1327962

J J McCormick, personal communication

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