pRS425 (ATCC® 77106)

Applications: YE-type (episomal) shuttle vectorshuttle vectorvector containing primer sites useful for sequencingvector permitting RNA synthesis in vitrovector permitting production of single-stranded DNAvector permitting visual detection of recombinants  /  Depositors: TW Christianson

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Designations pRS425
Depositors TW Christianson
Biosafety Level 1
Host
Shipped in E. coli XL-1 Blue
Vector Information
Size (kb): 6.849
Vector: pRS425 (phagemid)
Promoters: Promoter T7 (phi10)
Construction: pJS92, pRS305, pBluescript II SK+
Marker(s):LEU2,ampR
Construct size (kb): 6.849
Features: insert detection: lacZ'
marker(s): LEU2
marker(s): ampR
promoter: T3
promoter: T7 (phi10)
promoter: lac
replicon: 2 micron
replicon: f1
replicon: pMB1
MCS: KpnI...SacI
Applications
YE-type (episomal) shuttle vector
shuttle vector
vector containing primer sites useful for sequencing
vector permitting RNA synthesis in vitro
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.8; EcoRI--4.8, 2.1; XbaI--3.6, 3.2; PvuII--6.4, 0.5.
Contains the REP3 and FRT sequences necessary for high copy propagation in yeast.
In S. cerevisiae, the copy number is about 20 per haploid cell. In non-selective growth, plasmids are lost through mitotic segregation at rates in the range of 4.6 + or - 0.6% of progeny per doubling.
YE-type shuttle vector encoding the beta-galactosidase alpha peptide, permitting blue-white visual detection. Can be used to produce ssDNA. Contains promoters for in vitro RNA synthesis and priming sites useful for sequencing.
The order of the major features in this plasmid is: ori(f1) - lacZ - T7 promoter - MCS (KpnI-SacI) - T3 promoter - lacI - ori(pMB1) - ampR - ori(2 micron) - LEU2.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Christianson TW, et al. Multifunctional yeast high-copy-number shuttle vectors. Gene 110: 119-122, 1992. PubMed: 1544568

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Shipped frozen
Shipping Information Frozen glycerol stock of Escherichia coli XL-1 Blue containing the phagemid.
Product Sheet
Product Sheet
Product Sheet
Attachments Map of pRS425, ATCC 77106