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pcCSF-17 [CMCC 2347] (ATCC® 53149)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: Cetus Corp.

Permits and Restrictions

View Permits

Designations pcCSF-17 [CMCC 2347]
Species Homo sapiens, human
Depositors Cetus Corp.
Applications
in another host, produces protein colony stimulating factor 1 (macrophage)
Vector
Construct size (kb): 4.80
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pcD
Intact vector size: 3.000
Type of vector: plasmid
Cloning sites: XhoI BamHI
Polylinker sites:
Construction: pBR322, SV40
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
enhancer: SV40
marker(s): ampR
promoter: SV40 early, late
replicon: pMB1, SV40
terminator: SV40 late
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CSF1
Genomic copy number: unique
Gene name: colony stimulating factor 1 (macrophage)
Contains complete coding sequence?: U
Chromosome: 1; Localization: 1 p21-p13
Tissue: MIA PaCa-2 cell line
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 1.64
Cross references:
Insert lengths(kb): 1.64
Tissue: MIA PaCa-2 cell line
Gene product: colony stimulating factor 1 (macrophage) [CSF1]
Target Gene: colony stimulating factor 1 (macrophage)
Insert Size (kb) 1.640
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.8; HindIII--4.8; BamHI--3.0, 0.9, 0.87; PstI--3.0, 1.3, 0.35; SalI--4.8.
When this plasmid is transfected into COS cells, the product shows activity by radioreceptor assay, bone marrow proliferation assay, and CFU assay.
This insert encodes a mature CSF-1 protein of 224 amino acids. The insert includes approximately 180 bp 5' untranslated sequence and approximately 700 bp 3' untranslated sequence.
References

Kawasaki ES, et al. DNA encoding for CSF-1 and accompanying recombinant systems. US Patent 4,847,201 dated Jul 11 1989

Shadle PJ, et al. Conjugation of polymer to colony stimulating factor-1. US Patent 4,847,325 dated Jul 11 1989

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Bascic Documentation
U.S. Patent
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.