Escherichia coli (Migula) Castellani and Chalmers (ATCC® 47020)

Strain Designations: WM6  /  Type Strain: no  /  Biosafety Level: 1

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Classification Enterobacteriaceae, Escherichia
Strain Designations WM6
protease-deficient transformation host
transformation host
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Shipping Information

Shipped:  Freeze dried E. coli

Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Genotype F- supC(Ts) lac(Am) mal trp(Am) rpsL htpR165 pho(Am) lambda-
Type Strain no
The depositor indicated that this strain was derived from CAG-456. The genotype is not complete because the nature of the reversion is not known.
This strain has reduced proteolysis of foreign proteins. It does not require heat induction to reduce proteolysis and has the potential to accumulate foreign protein over a longer period of time.
Medium ATCC® Medium 1065: LB Agar/Broth, Miller
Growth Conditions
Temperature: 30.0°C
Genotype F- supC(Ts) lac(Am) mal trp(Am) rpsL htpR165 pho(Am) lambda-
Name of Depositor W Mandecki

Mandecki W, et al. High-level expression of a gene encoding the human complement facator C5a in Escherichia coli. Gene 43: 131-138, 1986. PubMed: 3530879

Baker TA, et al. A gene regulating the heat shock response in Escherichia coli also affects proteolysis. Proc. Natl. Acad. Sci. USA 81: 6779-6783, 1984. PubMed: 6387713

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation