Designation / Description PBGST7 PURIFIED PLASMID DNA
U.S. Patent Number
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.7, 0.6; EcoRI--3.4; HindIII--3.4.
Distributed in aliquots of 200 ng dried.
If linearized with HindIII, the T7 runoff transcript can be used to produce the ribozyme. The transcript is 716 nt long (274 nt of 5' exon, 413 nt of intervening sequence, and 29 nt of 3' exon).
Lengths of the exon sequences were adjusted so that accurate splicing in E. coli would permit expression of beta-galactosidase activity by alpha-complementation.
The vector was constructed by inserting the T7 promoter sequence into a HaeII site approximately 230 bp upstream of the multiple cloning site.
Shipped freeze-dried
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Basic Documentation

Cech TR, et al. RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods. US Patent 4,987,071 dated Jan 22 1991

Been MD, Cech TR. One binding site determines sequence specificity of Tetrahymena pre-rRNA self-splicing, trans-splicing, and RNA enzyme activity. Cell 47: 207-216, 1986. PubMed: 3021333