Size (kb): 8.83
Vector: pMMB67EH (plasmid)
Promoters: Promoter tac
Construction: pMMB66EH (ATCC 37620)
, M13mp18 polylinker
Construct size (kb): 8.83
Features: marker(s): ampR
replicon: RSF1010 (IncQ)
repressor gene: lacIq
transcription terminator: rrnB
Contains the following sites separated by (bp): EcoRI-785 -AhaIII-97 -ScaI-112 -PvuI-2101 -AccI-1848 -ScaI-55 -AccI-2039 -BstEII-372 -PvuII-93 -PvuII-94 -HpaI-320 -BstEII-182 -MluI-732 -EcoRI.
Restriction digests of the clone give the following sizes (kb): AccI--3.7, 3.1, 1.8; BamHI--8.8; HindIII/PvuII--7.2, 1.3. NOTE: A CONSTANT 3.0 KB BAND MAY BE OBSERVED. IT IS NOT A CONTAMINANT BUT APPEARS TO BE AN ANOMALY.
In the pMMB66 (ATCC 37620
, 37621) and pMMB67 (ATCC 37622
, 37623) vector series, the EH and HE in the name designate the orientation of the multiple cloning site to the tac promoter.
An autoregulated high-level expression vector utilizing the tac promoter, rrnB terminators and lacIq for lac repression. Concentration of induced gene product may be regulated by varying the lac inducer concentration.
Can be mobilized by conjugative IncP helper plasmids [e.g. pRK2013 (ATCC 37159)
, pUB307] into Klebsiella aerogenes, Proteus mirabilis, Pseudomonas aeruginosa, Serratia marcescens, and other gram-negative bacteria.
Compatible with common E. coli cloning vectors, as well as vectors derived from IncP and IncW plasmids.
The M13mp8 polylinker of pMMB66EH (ATCC 37620)
was replaced with the M13mp18 polylinker.
Belongs to a group of vectors (ATCC 37622-37623, 77287-77292) differing only in antibiotic resistance and polylinker orientation.
Furste JP, et al. Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector. Gene 48: 119-131, 1986. PubMed: 3549457
Jon A Kornacki, personal communication
Erich Lanka, personal communication
Shipped: Frozen glycerol stock of E. coli containing the plasmid.