Universal Mycoplasma Detection Kit
30-1012K ™
The Universal Mycoplasma Detection Kit offers a quick and sensitive PCR-based test to detect mycoplasma contaminants in cell culture. All components required for the PCR reaction are provided and have been optimized for amplification. High specificity is obtained through the utilization of a proprietary mix of buffers, dNTPs and thermostable polymerase, combined with universal primers that are specific to the 16S rRNA coding region in the mycoplasma genome. DNA originating from other sources, such as tissue samples or other bacteria, are not amplified. A touchdown PCR regimen increases sensitivity of the assay, along with enhancing specificity.
The kit detects over 60 species of Mycoplasma, Acholeplasma, Spiroplasma and Ureaplasma including the eight species most likely to afflict cell cultures: M. arginini, M. fermentans, M. hominis, M.hyorhinis, M. orale, M. pirum, M. salivarium, and A. laidlawii. Samples that are positive for mycoplasma are easily recognized by a distinct PCR product ranging in size from 434 to 468 bp on an agarose gel.
To access the detailed instruction sheet for this kit, click on the Protocol icon in the Documentation section of the product page.
Use promo code ATCC-000029 at checkout to save 25% on the kit.*
*This online-only offer is effective from April 1 –30, 2024. The 25% discount applies only to the ATCC Universal Mycoplasma Detection Kit; all other items are excluded from this promotion. The products received through this promotional offer will have an expiration date of June 30, 2024. For non-profit customers, the promotional discount is applied to the customer-specific pricing. The promo code must be applied at the time of checkout. The promo code must be applied in the “Promotional code” field in the shopping cart. U.S. customers only. Void where prohibited. Restrictions may apply. Limit 5 kits.