Staphylococcus epidermidis (Winslow and Winslow) Evans (ATCC® 12228)

Strain Designations: FDA strain PCI 1200  /  Type Strain: no  /  Biosafety Level: 1

Permits And Restrictions

View Permits

Strain Designations FDA strain PCI 1200
Application
Food testing
Pharmaceutical and Personal Care
Assay of gentamicin RefEuropean Pharmacopoeia Commission Microbiological assay of antibiotics. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 2.7.2, 1997 RefU.S. Pharmacopeia General Chapters; <81> Antibiotics-Microbial Assays. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011
Assay of neomycin RefU.S. Pharmacopeia General Chapters; <81> Antibiotics-Microbial Assays. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011 RefAOAC International Antibiotics in feeds, microbiological methods. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 957.23. Ref Neomycin in Feeds -- Stahl Microbiological Agar Diffusion Assay. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 998.02.
Assay of netilmycin RefU.S. Pharmacopeia General Chapters; <81> Antibiotics-Microbial Assays. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011
Assay of novobiocin RefU.S. Pharmacopeia General Chapters; <81> Antibiotics-Microbial Assays. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011RefAOAC International Antibiotics in feeds, microbiological methods. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 957.23.
Assay of oleandomycin
Assay of paromomycin aminosidine, catenulin RefU.S. Pharmacopeia General Chapters; <81> Antibiotics-Microbial Assays. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011
Assay of sisomicin RefU.S. Pharmacopeia General Chapters; <81> Antibiotics-Microbial Assays. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011
Inhibition testing Ref Standard Test Method for Evaluation of Antimicrobial Formulations by the Agar Patch Technique. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1882-05.
Media testing Ref Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - 3rd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M22-A3. Ref Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11133-2:2003. Ref Microbiology of food and animal feeding stuffs --- Guidelines on preparation and production of culture media --- Part 2: Practical guidelines on performance testing of culture media - Annex B: Recommended test microorganisms for commonly used culture media. London, UK:British Standards Institution;British Standard DD CEN ISO/TS 11133:2003.
Quality control strain Ref Applied Biosystems, MicroSeq Pharmaceutical Validation Panel. Ref BD Crystal Gram-Positive QC Set. BD Becton Dickinson; Crystal  Ref Applied Biosystems, MicroSeq PHL Panel.
Sterility testing
Susceptibility disc testing neomycin
Susceptibility disc testing vancomycin RefZhang YQ, et al. Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228). Mol. Microbiol. 49: 1577-1593, 2003. PubMed: 12950922
Type Strain no
Biosafety Level 1
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® yes
Genome Sequenced Strain

Staphylococcus epidermidis ATCC 12228, complete genome.

Type Strain no
Comments
Genome sequenced strain
Strain does not form a biofilm RefZhang YQ, et al. Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228). Mol. Microbiol. 49: 1577-1593, 2003. PubMed: 12950922
Sensitive to vancomycin RefZhang YQ, et al. Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228). Mol. Microbiol. 49: 1577-1593, 2003. PubMed: 12950922
Medium ATCC® Medium 3: Nutrient agar or nutrient broth
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Name of Depositor FDA
References

British Pharmacopoeia Commission Biological assay of antibiotics. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XIV A, 2003

European Pharmacopoeia Commission Microbiological assay of antibiotics. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 2.7.2, 1997

Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - 3rd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M22-A3.

U.S. Pharmacopeia General Chapters; <81> Antibiotics-Microbial Assays. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011

GEN III MicroPlate™ QC/ OmniLog® & MicroStation™ Validation Set

AOAC International Antibiotics in feeds, microbiological methods. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 957.23.

Applied Biosystems, MicroSeq Pharmaceutical Validation Panel.

BD Crystal Gram-Positive QC Set. BD Becton Dickinson; Crystal

Neomycin in Feeds -- Stahl Microbiological Agar Diffusion Assay. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 998.02.

Efficacy of Preservation on Non-Eye Area Water-Miscible Cosmetic and Toiletry Formaulations. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 998.10.

Microbial assay for antibiotics. Tokyo, Japan:Japanese Pharmacopoeia;JP JP14e.part I.34.

Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11133-2:2003.

Applied Biosystems, MicroSeq PHL Panel.

Standard Test Method for Evaluation of Antimicrobial Formulations by the Agar Patch Technique. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1882-05.

Microbiology of food and animal feeding stuffs --- Guidelines on preparation and production of culture media --- Part 2: Practical guidelines on performance testing of culture media - Annex B: Recommended test microorganisms for commonly used culture media. London, UK:British Standards Institution;British Standard DD CEN ISO/TS 11133:2003.

VITEK 2 BCL Comprehensive QC Set. bioMerieux.

VITEK 2 YST Comprehensive QC Set. bioMerieux.

VITEK 2 Comprehensive QC Set. bioMerieux.

BD Crystal Rapid Gram-Positive QC Set. BD Becton Dickinson.

ISO/CD 11133:2009, Annex E. Test microorganisms for commonly used culture media (giving information on the culture medium, culture conditions, test microorganisms, culture collection number of test organisms and the expected reactions)

Corry, JEL, Curtis, GDW and Baird, RM (Eds) Handbook of Culture Media for Food and Water Microbiology. Royal Society of Chemistry, In preparation. ICFMH-WPCM.

Zhang YQ, et al. Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228). Mol. Microbiol. 49: 1577-1593, 2003. PubMed: 12950922

BD MAX™ StaphSR Assay. BD Becton Dickinson.

Cross References

Nucleotide (GenBank) : U39771 Staphylococcus epidermidis 16S-23S ribosomal RNA spacer region.

Nucleotide (GenBank) : U90018 Staphylococcus epidermidis 16S-23S ribosomal RNA spacer region.

Nucleotide (GenBank) : U90019 Staphylococcus epidermidis 16S-23S ribosomal RNA spacer region.

Nucleotide (GenBank) : U90020 Staphylococcus epidermidis 16S-23S ribosomal RNA spacer region.

Nucleotide (GenBank) : U90021 Staphylococcus epidermidis 16S-23S ribosomal RNA spacer region.

Nucleotide (GenBank) : AE015929 Staphylococcus epidermidis ATCC 12228, complete genome.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation