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Candida utilis (Henneberg) Lodder et Kreger-van Rij

9950-MINI-PACK

ATCC® 9950-MINI-PACK™ consists of 6 ready-to-use vials of ATCC® 9950™ frozen in 200 µL of glycerol stock, eliminating the need to rehydrate and culture the strain prior to use. Each vial is provided with a 2-D barcode for easy storage and tracking, as well as peel-off labels for fast and reliable recordkeeping.
Product category
Fungi
Product type
Yeast
Strain designation
NRRL Y-900 [CBS 5609, CCRC 20325, DSM 2361, IFO 0988, NBRC 0988, NCYC 707, NRCC 2721, VTT C-78085]
Type strain
No
Genome sequenced strain
Yes
Applications
Bioremediation
Quality control
Product format
Frozen
Shipping information
6 ready-to-use vials containing the strain in glycerol stock
Storage conditions
-80°C or colder
Mission Collection Item
This is a Mission Collection Item

Documentation

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Biosafety Icon BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
ATCC® 9950-MINI-PACK™ consists of 6 ready-to-use vials of ATCC® 9950™ frozen in 200 µL of glycerol stock, eliminating the need to rehydrate and culture the strain prior to use. Each vial is provided with a 2-D barcode for easy storage and tracking, as well as peel-off labels for fast and reliable recordkeeping. Developed by the leaders in microbial cultivation and preservation, ATCC® Minis provide a convenient, ready-to-use solution for handling quality control strains. ATCC® Minis are authenticated and backed by ATCC polyphasic testing – ensuring the same consistent and reliable reference materials you’ve come to trust for ATCC Genuine Cultures®. It is easy to ensure the quality of your products with ATCC® Minis – just open, plate, and go!

Accumulates zinc
Assay of antimicrobial agent
Degrades RNA ribonucleic acid
Degrades alfalfa process wastes
Degrades ethyl alcohol ethanol
Degrades pickle process brine
Degrades potato wastes
Degrades rapeseed oil meal
Degrades sauerkraut brine
Produces S-adenosylmethionine
Produces acetaldehyde
Produces ethyl acetate
Produces methionine adenosyltransferase S-adenosylmethionine synthetase
Produces single-cell protein SCP
Quality control strain
Transformation host
Transformation host for expression of carotenogenic genes
Used in Controlled Ecological Life Support Systems (CELSS) for food
Produces adenosyl-D-methionine and adenosyl-2-methylmethionine
Produces high quality edible protein
Conversion of 5'-methylthioadenosine into S-adenosylmethionine
Preceptrol
No

Characteristics

Comments
Cell vacuoles
Genome sequencing strain (KIRIN Holdings Company, Japan)

Handling information

Medium
Temperature
25-30°C
Atmosphere
Aerobic
Handling procedure
Frozen mini-cryovials packed in dry ice should either be thawed immediately for use or stored at or below
-70°C until the expiration date printed on the label. Storage at -20°C may affect the growth and viability of the culture.

  1. To thaw a frozen mini-cryovial, place the vial upright in a 25°C to 30°C water bath, until just thawed (approximately 2-3 minutes).  Immerse the mini-cryovial just sufficient to cover the frozen material.  Do not agitate the mini-cryovial.
  2. Immediately after thawing, wipe down the mini-cryovial with 70% ethanol and aseptically transfer at least 50 µL (or 2-3 agar cubes) of the content onto a plate or broth with the recommended medium.
  3. Discard the empty vial. Do not refreeze any unused portion as it will result in a loss of viability.
  4. Incubate the inoculum/strain at the temperature and conditions recommended. Inspect for growth of the inoculum/strain regularly. Viability is typically noticeable after 2-3 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
Handling notes
Additional, updated information on this product may be available on the ATCC® web site at www.atcc.org.

History

Deposited as
Torulopsis utilis (Henneberg) Lodder
Depositors
NRRL
Special collection
ATCC® Minis
Cross references
GenBank AJ277098 Candida utilis anc gene for mitochondrial ADP/ATP carrier, isoform
GenBank AJ277099 Candida utilis anc gene for mitochondrial ADP/ATP carrier, isoform

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Lawford GR, et al. Production of high-quality edible protein from Candida yeast grown in continuous culture. Biotechnol. Bioeng. 21: 1163-1174, 1979.

Maul SB, et al. New process for reducing the nucleic acid content of yeast. Nature 228: 181, 1970. PubMed: 5460024

Ohta S, et al. Characterization of a heat-shock process for reduction of the nucleic acid content of Candida utilis. Appl. Microbiol. 22: 415-421, 1971. PubMed: 4330316

Brewer D, et al. The antibiotic activity of cultures from fungal spores collected by a spore trap on permanent pasture. Can. J. Microbiol. 20: 721-729, 1974. PubMed: 4857622

Hang YD, et al. Sauerkraut waste: a favorable medium for cultivating yeasts. Appl. Microbiol. 24: 1007-1008, 1972.

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