Afipia felis Brenner et al.
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2. Using a single tube of #1099 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate an agar slant and/or plate.
5. Incubate at 30oC for three to four days.
Cells are Gram negative, very thin, medium-sized, pleomorphic rods.
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Validation list No. 41. Int. J. Syst. Bacteriol. 42: 327-329, 1992.
O'Connor SP, et al. 16S rRNA sequences of Bartonella bacilliformis and cat scratch disease bacillus reveal phylogenetic relationships with the alpha-2 subgroup of the class Proteobacteria. J. Clin. Microbiol. 29: 2144-2150, 1991. PubMed: 1719021
Brenner DJ, et al. Proposal of Afipia gen. nov., with Afipia felis sp. nov. (formerly the cat scratch disease bacillus), Afipia clevelandensis sp. nov. (formerly the Cleveland Clinic Foundation strain), Afipia broomeae sp. nov., and three unnamed genospecies. J. Clin. Microbiol. 29: 2450-2460, 1991. PubMed: 1774249