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Helicobacter pylori (Marshall et al.) Goodwin et al.

49503

Product category
Bacteria
Strain designation
60190
Type strain
No
Applications
Enteric disease research
Infectious disease research
Product format
Frozen
Storage conditions
-80°C or colder
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Biosafety Icon BSL 2

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Enteric Research
Preceptrol
No

Characteristics

Comments
Cytotoxin activity and serologic response
Technical information
ATCC Technical Services does not have technical information on patent deposits that are not produced or characterized by ATCC. Additional information can be found in the corresponding patent available from the patent holder or with the U.S. and/or international patent office.

Handling information

Medium
Temperature
37°C
Atmosphere
Microaerophilic
Handling procedure
  1. Just prior to use, thaw vial in water for 2-3 minutes at approximately 37°C.
  2. Open thawed vial according to enclosed instructions or visit www.atcc.org for instructions.
  3. Aseptically transfer the entire contents to a 5-6 mL tube of #2856 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes.
  4. Use several drops of the primary broth tube to inoculate a #2856 plate and/or #2856 agar slant.
  5. Or, to obtain a biphasic culture, add several drops of the primary broth tube to a #2856 agar slant. Best practice is to incubate these slants at an angle.
  6. Incubate at 37°C under microaerophilic conditions for 3 days. Use an anaerobe jar with an active catalyst and a microaerophilic gas generator pack or other acceptable method. All tubes and slants should be incubated with caps loosened.
Handling notes
This strain grows more slowly than most other Helicobacter pylori and requires moist conditions for best growth. Growth at the broth/agar interface of the biphasic slant should occur within 3 days, but only slight turbidity will be seen. To observe growth, examine a wet mount of the broth under phase microscopy.
The cells do not Gram stain well using traditional procedures. For best results, use a basic fuchsin counterstain in place of the safranin.
Once good growth in obtained, transfer or freeze the culture. Adding an equal amount of 20% sterile glycerol to pooled broth from several biphasic slants, followed by freezing in liquid nitrogen or "ultra-low temperature" freezer is recommended.
Additional information on this culture is available on the ATCC® web site at www.atcc.org.

History

Deposited as
Helicobacter pylori (Marshall et al.) Goodwin et al.
Depositors
TL Cover
Chain of custody
ATCC <-- TL Cover <-- R. Leunk <-- A. Pearson and P. Hawtin
Patent depository
This material was deposited with the ATCC Patent Depository to fulfill U.S. or international patent requirements. This material may not have been produced or characterized by ATCC.  As an International Depository Authority (IDA) for patent deposits, ATCC is required to complete viability testing only at time of initial deposit of patent material. Patent deposits are made available on behalf of the Depositor when the pertinent U.S. or international patent is issued, but material may not be used to infringe the patent claims.
Patent number
5,618,840
Cross references
GenBank Y08762 Helicobacter pylori flhA gene and unknown ORF.
GenBank AC002058 Helicobacter pylori, *** SEQUENCING IN PROGRESS ***, in ordered pieces.
GenBank U05676 Helicobacter pylori 60190 cysteinyl-tRNA synthetase homolog gene, partial cds, and vacuolating cytotoxin (vacA) gene, complete cds.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Disclosures
This material is cited in a US and/or international patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Depositor of the party to which the material was furnished.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Cover TL, et al. Characterization of and human serologic response to proteins in Helicobacter pylori broth culture supernatants with vacuolizing cytotoxin activity. Infect. Immun. 58: 603-610, 1990. PubMed: 2307514

Cao P, Cover TL. High-level genetic diversity in the vapD chromosomal region of Helicobacter pylori. J. Bacteriol. 179: 2852-2856, 1997. PubMed: 9139899

Melchers K, et al. Cloning and membrane topology of a P type ATPase from Helicobacter pylori. J. Biol. Chem. 271: 446-457, 1996. PubMed: 8550601

Dubois A, et al. Transient and persistent experimental infection of nonhuman primates with Helicobacter pylori: implications for human disease. Infect. Immun. 64: 2885-2891, 1996. PubMed: 8757808

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