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NGS-Ready DNA from Escherichia coli strain CDC B6914-MS1 serotype O157-H7

43888DX

NGS-ready DNA extracted from Escherichia coli, strain CDC B6914-MS1 [serotype O157:H7] [ATCC® 43888™].
Product category
Bacteria
Product type
Nucleic acid
Derived from
Escherichia coli CDC B6914-MS1 (ATCC 43888)
Organism
Escherichia coli (Migula) Castellani and Chalmers
Type strain
No
Genome sequenced strain
Yes
Applications
Bioinformatics
Next-generation sequencing
Infectious disease research
Enteric disease research
Mass
10 μg
Product format
Frozen
Shipping information
DNA in 10 mM Tris-HCl buffer, pH 8.5
Fragment size ≥ 20 kb
Storage conditions
-20°C or colder
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Price: $650.00 ea
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Biosafety Icon BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
ATCC® NGS-Ready DNA is carefully extracted to produce concentrated, purified, long fragment, high-molecular-weight DNA required for Next Generation Sequencing platforms. ATCC® NGS-Ready DNA has been functionally verified and the resultant sequence passes stringent qualifications reported on the Certificate of Analysis for each lot. Sequence produced from this product is available through the ATCC® Genome Portal (genomes.atcc.org).

Characteristics

Comments
Genomic DNA extracted from a preparation of Escherichia coli, strain CDC B6914-MS1 [serotype O157:H7] (ATCC® 43888™). The source organism is also available through the ATCC catalog.

Handling information

Handling procedure
  1. Thaw the vial at room temperature and immediately place on ice. Avoid exposing the DNA to repeated freeze-thaw cycles as it may result in degradation. If the complete vial will not be used for your application, aliquoting is highly recommended to avoid multiple freeze-thaws.
  2. Gently mix the sample to ensure an even distribution of material. Avoid vortex use and repeat pipetting to maintain DNA.
  3. Briefly centrifuge the tube before opening to ensure all liquid is at the bottom.
Handling notes
Sequence produced from this product is available through the ATCC® Genome Portal (genomes.atcc.org).

Quality control specifications

Electrophoresis - molecular weight
≥20 kilobase pairs
Total amount
Total DNA by PicoGreen® measurement: ≥ 10 μg
Functional tests
Functional activity confirmed by whole-genome sequencing
Identity
Identity confirmed by data analyses performed using ONE CODEX®.
Verification method
Whole-genome Sequencing

History

Depositors
ATCC

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Frequently Asked Questions

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