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Helicobacter pylori (Marshall et al.) Goodwin et al.

43504

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Type strain. Quality control strain for API products. 
Product category
Bacteria
Strain designation
NCTC 11637 [JCM 7653, RPH 13487]
Type strain
Yes
Isolation source
Gastric antrum
Geographical isolation
Australia
Applications
Bioinformatics
Enteric disease research
Infectious disease research
Media testing
Quality control
Product format
Frozen
Storage conditions
-80°C or colder
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Biosafety Icon BSL 2

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Media testing
Quality control strain
Susceptibility testing
Quality control strain for API products
Enteric Research
Preceptrol
No

Handling information

Medium
Temperature
37°C
Atmosphere
Microaerophilic: 3-5% O2, 10% CO2
Handling procedure
  1. Open thawed vial according to enclosed instructions or visit www.atcc.org for instructions.
  2. Aseptically transfer the entire contents to a 5-6 mL tube of #18 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes.
  3. Use several drops of the primary broth tube to inoculate a #260 plate and/or #260 agar slant.
  4. Or, to obtain a biphasic culture, add 0.2-0.5 mL of the primary broth tube to a #260 agar slant. Best practice is to incubate these slants at an angle.
  5. Incubate at 37°C under microaerophilic conditions for 3-5 days. Use an anaerobe jar with an active catalyst and a microaerophilic gas generator pack or other acceptable method. All tubes and slants should be incubated with caps loosened.
Handling notes

Purified genomic DNA of this strain is available as ATCC® 43504D-5™.

Additional information on this culture is available on the ATCC® web site at www.atcc.org.

Quality control specifications

Verification method
Whole-genome Sequencing

History

Deposited as
Campylobacter pyloridis Marshall et al.
Depositors
NCTC
Chain of custody
ATCC <-- NCTC <-- CS Goodwin and DI Annear <-- H Royce
Type of isolate
Human
Special collection
NCRR Contract
Cross references
GenBank A92782 Sequence 20 from Patent WO9804702.
GenBank A92783 Sequence 21 from Patent WO9804702.
GenBank A92784 Sequence 22 from Patent WO9804702.
GenBank Y18985 Helicobacter pylori hopZ gene, strain ATCC 43504.
GenBank Y18995 Helicobacter pylori hopV gene, strain ATCC 43504.
GenBank Y18996 Helicobacter pylori hopW gene, strain ATCC 43504.
GenBank Y18997 Helicobacter pylori hopX gene, strain ATCC 43504.
GenBank Y18998 Helicobacter pylori hopZ gene, strain ATCC 43504.
GenBank U01330 Helicobacter pylori isolate ATCC 43504 16S rRNA gene.
GenBank M88157 Helicobacter pylori (ATCC 43504) partial 16S ribosomal RNA.
GenBank U68082 Helicobacter pylori coded portion of proteolysis tag tmRNA gene.
GenBank AF053357 Helicobacter pylori glutamine synthetase (glnA) gene, complete cds.
GenBank AF053715 Helicobacter pylori GTP binding protein RgbA (rgbA) gene, partial cds.
GenBank AF010307 Helicobacter pylori AbcA (abcA), AbcB (abcB), AbcC (abcC) and AbcD (abcD) genes, complete cds.
GenBank AF125214 Helicobacter pylori cag pathogenicity island CagA (cagA) gene, partial cds; and urease-enhancing factor Lpp (lpp), urease-enhancing factor helB (helB), and HelA (helA) genes, complete cds.
GenBank AF125197 Helicobacter pylori 3-dehydroquinase type II AroQ (aroQ) gene, partial cds; hypothetical protein, small ribosomal subunit protein 15 RpsL15 (rpsL15), and flagellar biosynthesis/regulation protein FlbA (flbA) genes, complete cds; and hypoth

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Validation list no. 17. Int. J. Syst. Bacteriol. 35: 223-225, 1985.

Marshall BJ, Goodwin CS. Revised nomenclature of Campylobacter pyloridis. Int. J. Syst. Bacteriol. 37: 68, 1987.

Fox JG, et al. Campylobacter pylori subsp. mustelae subsp. nov. isolated from the gastric mucosa of ferrets (Mustela putorius furo), and an emended description of Campylobacter pylori. Int. J. Syst. Bacteriol. 38: 367-370, 1988.

Goodwin CS, et al. Transfer of Campylobacter pylori and Campylobacter mustelae to Helicobacter gen. nov. as Helicobacter pylori comb. nov. and Helicobacter mustelae comb. nov., respectively. Int. J. Syst. Bacteriol. 39: 397-405, 1989.

Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard - 9th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M2-A9.

View All Curated Citations for this Product

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