Corynebacterium glutamicum (Kinoshita et al.) Abe et al.
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2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate an additional broth tube, a #3 agar slant and/or a plate.
5. Incubate all tubes and plate at 30°C for 24-48 hours.
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Nakayama K, Hagino H. Process for producing L-tryptophan. US Patent 3,849,251 dated Nov 19 1974
Herry DM, Dunican LK. Cloning of the trp gene cluster from a tryptophan-hyperproducing strain of Corynebacterium glutamicum: identification of a mutation in the trp leader sequence. Appl. Environ. Microbiol. 59: 791-799, 1993. PubMed: 7683184