Corynebacterium glutamicum (Kinoshita et al.) Abe et al.
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2. Using a single tube of recommended broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or glass pipet. Rehydrate the pellet.
3. Transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a second tube of broth, a slant, and/or plate.
5. Incubate all tubes and plate at 30oC for 24 to 48 hours.
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Kubota K, et al. Method of producing L-lysine by fermentation. US Patent 3,825,472 dated Jul 23 1974
Colon GE, et al. Effect of inducible thrB expression on amino acid production in Corynebacterium lactofermentum ATCC 21799. Appl. Environ. Microbiol. 61: 74-78, 1995. PubMed: 7887627
derived from ATCC 13869