Corynebacterium glutamicum (Kinoshita et al.) Abe et al.
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2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #3 agar slant and/or plate.
5. Incubate the tubes and plate at 30°C for 24 hours.
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Nakayama K, Araki K. Process for producing L-histidine. US Patent 3,713,977 dated Jan 30 1973
derived from ATCC 13032