Corynebacterium glutamicum (Kinoshita et al.) Abe et al.
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2. Using a single tube of recommended broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or glass pipet. Rehydrate the pellet.
3. Transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a second tube of broth, a slant, and/or plate.
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Inuzuka K, Hamada S. Process for the production of L-lysine. US Patent 3,959,075 dated May 25 1976