Streptomyces griseus subsp. griseus (Krainsky) Waksman and Henrici
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
2. Using a single tube of #1877 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #196 agar slant and/or plate.
5. Incubate the tubes and plate at 26oC for 7 to 10 days.
Additional information on this culture is available on the ATCC web site at www.atcc.org.
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Bayan AP, et al. Antibiotic and vitamin B12 production by a steroid oxidizing actinomycete. J. Bacteriol. 69: 347-352, 1955. PubMed: 14367285
Perlman D, et al. 16 alpha-Hydroxyprogesterone, 16 alpha-hydroxydihydroprogesterone and esters thereof. US Patent 2,709,705 dated May 31 1955
Fried J, et al. 16 alpha-hydroxylation steroids. US Patent 2,855,343 dated Oct 7 1958
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.
Shirling EB, Gottlieb D. Cooperative description of type cultures of Streptomyces. III. Additional species descriptions from first and second studies. Int J Syst Bacteriol 18: 279-392, 1968.