Cell Immortalization

Green and blue epithelial htert cells.

Extend the lifespan of your cells

Custom Cell Immortalization

Many cell immortalization techniques can provide cell lines with extended proliferative capacity in vitro, while closely mimicking the physiology of cells in vivo. Unlike primary cells, immortalized cells are not susceptible to replicative senescence, reducing the need to repurchase and re-initiate growth, saving time and expense. ATCC offers cell immortalization services using various technologies such as human telomerase reverse transcriptase (hTERT), which provides stable genotypes of diverse cell types and tissue sources. Immortalized cell lines have an extended lifespan and typically are karyotypically, morphologically, and phenotypically similar to the primary parent cells.

What we offer our customers:

  • Custom immortalization services produce cell lines that have an extended lifespan and typically are similar to the primary parent cells
  • The ability to overcome the limited lifespan of primary cells by combining our custom cell derivation service with our custom cell immortalization service
  • In addition to standard ATCC cell authentication testing, immortalized cell lines can be tested for extended proliferative capacity and selected phenotypic markers from the tissue of interest (eg, the continuous expression of hTERT)
  • Experienced project managers and subject matter experts will work with you to produce an immortalized cell line tailored to your requirements

Custom Cell Immortalization

Contact us using the form below for more information on our services, including cell immortalization, or to let us know that you are ready to start your project.

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Thank you for your interest in our services!
Blue and green metastatic carcinoma cells.

Cell Engineering

Cell engineering involves physical and engineering principles to control cell behavior. At ATCC, we utilize a number of cell engineering processes including hTERT immortalization and CRISPR/Cas9 gene editing for site-specific genome engineering of cell lines.

Green and red kidney cells. Application note

hTERT-immortalized and Primary Keratinocytes Differentiate into Epidermal Structures in 3D Organotypic Culture

In this study, we confirmed that an immortalized keratinocyte cell line, Ker-CT, is able to differentiate into organotypic skin equivalents in a 3D culture model

ATCC resource Application note

ATCC Human Bronchial / Tracheal Epithelial Cells: Improving Functional Studies

This study will test the ability of primary human bronchial/tracheal epithelial cells (HBECs) to differentiate into mature airway tissue using an air-liquid culture interface (ALI) model.