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Clostridioides difficile (Prevot) Lawson et al. (ATCC® 9689-FZ™)

Strain Designations: 90556-M6S  /  Biosafety Level: 2

Deposited As Clostridium difficile (Hall and O'Toole) Prevot
Strain Designations 90556-M6S
Application
Produces cytotoxin
Enteric Research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format frozen
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Genotype Toxinotype: 0
Comments
Presence of tcdA and tcdB genes confirmed by PCR. RefLemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303
Binary toxin gene cdtB not amplified by PCR
Toxinotype 0
Ribotype 001
Medium ATCC® Medium 2107: Modified Reinforced Clostridial Agar/Broth Medium (pre-reduced)
ATCC® Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Growth Conditions Temperature:  37°C
Atmosphere:  Anaerobic gas mixture, 80% N2‑10% CO2‑10% H2
Genotype Toxinotype: 0
Name of Depositor FE Clark
Special Collection NSF - Bacteriology
Chain of Custody ATCC <-- F E Clark
References

Rolfe RD, Finegold SM. Purification and characterization of Clostridium difficile toxin. Infect. Immun. 25: 191-201, 1979. PubMed: 478634

Florin I, Thelestam M. Intoxication of cultured human lung fibroblasts with Clostridium difficile toxin. Infect. Immun. 33: 67-74, 1981. PubMed: 6167521

Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303

Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999

Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986

Cross References

Nucleotide (GenBank) : X94328 C.difficile spo0A gene.

Nucleotide (GenBank) : AX110003 Sequence 736 from Patent WO0123604.

Nucleotide (GenBank) : AX110007 Sequence 740 from Patent WO0123604.

Nucleotide (GenBank) : AX110095 Sequence 828 from Patent WO0123604.

Nucleotide (GenBank) : AX111484 Sequence 2217 from Patent WO0123604.

Nucleotide (GenBank) : AX111505 Sequence 2238 from Patent WO0123604.

Nucleotide (GenBank) : AX111525 Sequence 2258 from Patent WO0123604.

Nucleotide (GenBank) : U36481 Clostridium difficile sporulation protein homolog (spo0A) gene, partial cds.

Nucleotide (GenBank) : S51968 {16S/23S ribosomal DNA spacer region} [Clostridium difficile, ATCC 9689, Genomic, 271 nt]

Nucleotide (GenBank) : U43514 Clostridium difficile putative spoIVB (spoIVB) gene, complete cds, and phosphorylation-activated transcription factor spo0A (spo0a) gene, complete cds.