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1.800.638.6597

Clostridioides difficile (Prevot) Lawson et al. (ATCC^® 9689-FZ^™)

Strain Designations: 90556-M6S / Biosafety Level: 2

Clostridioides difficile (Prevot) Lawson et al. ATCC^® 9689-FZ^™

frozen

For-Profit: $400.00 Non-Profit: $340.00

Qty:

Permits and Restrictions	View Permits
Deposited As	Clostridium difficile (Hall and O'Toole) Prevot
Strain Designations	90556-M6S
Application	Produces cytotoxin Enteric Research
Biosafety Level	2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format	frozen
Storage Conditions	Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section

Preceptrol®	no
Genotype	Toxinotype: 0
Comments	Presence of tcdA and tcdB genes confirmed by PCR. RefLemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303 Binary toxin gene cdtB not amplified by PCR Toxinotype 0 Ribotype 001

Medium	ATCC^® Medium 2107: Modified Reinforced Clostridial ATCC^® Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Growth Conditions	Temperature: 37°C Atmosphere: Anaerobic gas mixture, 80% N₂‑10% CO₂‑10% H₂

Genotype	Toxinotype: 0

Name of Depositor	FE Clark
Special Collection	NSF - Bacteriology
Chain of Custody	ATCC <-- F E Clark
References	Rolfe RD, Finegold SM. Purification and characterization of Clostridium difficile toxin. Infect. Immun. 25: 191-201, 1979. PubMed: 478634 Florin I, Thelestam M. Intoxication of cultured human lung fibroblasts with Clostridium difficile toxin. Infect. Immun. 33: 67-74, 1981. PubMed: 6167521 Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980. Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303 Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999 Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986
Cross References	Nucleotide (GenBank) : X94328 C.difficile spo0A gene. Nucleotide (GenBank) : AX110003 Sequence 736 from Patent WO0123604. Nucleotide (GenBank) : AX110007 Sequence 740 from Patent WO0123604. Nucleotide (GenBank) : AX110095 Sequence 828 from Patent WO0123604. Nucleotide (GenBank) : AX111484 Sequence 2217 from Patent WO0123604. Nucleotide (GenBank) : AX111505 Sequence 2238 from Patent WO0123604. Nucleotide (GenBank) : AX111525 Sequence 2258 from Patent WO0123604. Nucleotide (GenBank) : U36481 Clostridium difficile sporulation protein homolog (spo0A) gene, partial cds. Nucleotide (GenBank) : S51968 {16S/23S ribosomal DNA spacer region} [Clostridium difficile, ATCC 9689, Genomic, 271 nt] Nucleotide (GenBank) : U43514 Clostridium difficile putative spoIVB (spoIVB) gene, complete cds, and phosphorylation-activated transcription factor spo0A (spo0a) gene, complete cds.

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis SDS