Escherichia coli (Migula) Castellani and Chalmers (ATCC® BAA-2199™) Strain Designations: 2000-3281 / Type Strain: no / Biosafety Level: 2 General Information Characteristics Culture Method History Documentation Print Email Share Share this product Facebook Twitter Google+ Classification Enterobacteriaceae, Escherichia Strain Designations 2000-3281 Application Enteric ResearchFood testing Isolation Ohio Biosafety Level 2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. Antigenic Properties Serotype O103:H25 Product Format freeze-dried Storage Conditions Frozen: -80°C or colderFreeze-Dried: 2°C to 8°CLive Culture: See Propagation Section Preceptrol® no Type Strain no Antigenic Properties Serotype O103:H25 Comments This strain was confirmed by PCR to carry the eae and stx1 genes (stx1+, stx2-, eae+). Toxigenic Depositor-provided information: Shiga toxin +, genotype ipaH-, stx1+, stx2-, eae+, E-hly+ Medium ATCC® Medium 3: Nutrient agar or nutrient broth Growth Conditions Temperature: 37°CAtmosphere: Aerobic Name of Depositor N Strockbine Isolation Ohio References Nancy A Strockbine, personal communication Basic Documentation Product Sheet Certificate of Analysis SDS