Escherichia coli (Migula) Castellani and Chalmers (ATCC® BAA-2199™) Strain Designations: 2000-3281 / Type Strain: no / Biosafety Level: 2 General Information Characteristics Culture Method History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits and Restrictions View Permits Classification Enterobacteriaceae, Escherichia Strain Designations 2000-3281 Application Enteric ResearchFood testing Isolation Ohio Biosafety Level 2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. Antigenic Properties Serotype O103:H25 Product Format freeze-dried Storage Conditions Frozen: -80°C or colderFreeze-Dried: 2°C to 8°CLive Culture: See Propagation Section Preceptrol® no Type Strain no Antigenic Properties Serotype O103:H25 Comments This strain was confirmed by PCR to carry the eae and stx1 genes (stx1+, stx2-, eae+). Toxigenic Depositor-provided information: Shiga toxin +, genotype ipaH-, stx1+, stx2-, eae+, E-hly+ Medium ATCC® Medium 3: Nutrient agar or nutrient broth Growth Conditions Temperature: 37°CAtmosphere: Aerobic Name of Depositor N Strockbine Isolation Ohio References Nancy A Strockbine, personal communication Permits These permits may be required for shipping this product: This item is listed on the Commerce Control List of the U.S. Department of Commerce and is distributed only within the 50 United States. Distribution requires completion of a Customer Acceptance of Responsibility (CAR) for Commerce Control List (CCL) Biologicals form. Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis SDS