Escherichia coli (Migula) Castellani and Chalmers (ATCC® BAA-181)

Strain Designations: CDC 1999-3249  /  Type Strain: no  /  Biosafety Level: 2

Classification Enterobacteriaceae, Escherichia
Strain Designations CDC 1999-3249
Application
Produces Shiga toxin 1
Produces Shiga toxin 2
Produces verocytotoxin 1
Produces verocytotoxin 2
Enteric Research
Food testing
Isolation Stool from a human with HUS, South Dakota, 1999
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Antigenic Properties Serotype O111:H8
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Type Strain no
Antigenic Properties Serotype O111:H8
Comments This strain was confirmed by PCR to carry the eae, stx1, and stx2 genes (stx1+, stx2+, eae+).
Toxigenic

Growth on Novobiocin (10 mg/L)
Growth on Tellurite (0.8 mg/L)
Medium ATCC® Medium 18: Trypticase Soy Agar/Broth
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Name of Depositor NA Strockbine
Special Collection NCRR Contract
Isolation Stool from a human with HUS, South Dakota, 1999
References

Nancy A Strockbine, personal communication