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1.800.638.6597

CCRF-CEM [CCRF CEM] (ATCC^® CRM-CCL-119^™)

Organism: Homo sapiens, human / Cell Type: T lymphoblast (KRAS CRM) / Tissue: peripheral blood / Disease: acute lymphoblastic leukemia

CCRF-CEM [CCRF CEM] ATCC^® CRM-CCL-119^™

frozen

For-Profit: $714.00 Non-Profit: $606.90

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Permits and Restrictions	View Permits
Organism	Homo sapiens, human
Tissue	peripheral blood
Cell Type	T lymphoblast (KRAS CRM)
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	acute lymphoblastic leukemia
Age	4 years juvenile
Gender	female
Ethnicity	Caucasian
Applications	For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation. ISO Guide 34:2009 This line may be used as a transfection host
Intended Use	Certified Reference Material produced under an ISO Guide 34:2009 accredited process.
Storage Conditions	liquid nitrogen vapor phase

Karyotype	modal number = 47; range = 41 to 95. Fifty karyotypes showed no consistent loss or gain of particular chromosomes. Twenty-eight percent of the cells with 45 chromosomes were C-; 53% of all cells had an extra D and 35% had an extra F. Only N1 and N18 were not affected by gain or loss. No marker chromosomes noted.
Clinical Data	Caucasian female 4 years juvenile
Antigen Expression	CD3; Homo sapiens, expressed CD4; Homo sapiens, expressed CD5; Homo sapiens, expressed CD7; Homo sapiens, expressed
Genes Expressed	CD3; Homo sapiens, expressed ,CD5; Homo sapiens, expressed, CD7; Homo sapiens, expressed, CD4; Homo sapiens, expressed
Tumorigenic	Yes
Effects	Yes, in syrian hamsters RefAdams RA, et al. Leukemia: serial transplantation of human leukemic lymphoblasts in the newborn Syrian hamster. Cancer Res. 27: 772-783, 1967. PubMed: 4295047
Comments	Certificates of Analysis are available electronically at www.atcc.org, or by hardcopy upon request. Certified Reference Material produced under an ISO Guide 34:2009 accredited process. Item has been tested for KRAS mutation (p.G12D c.35G>A)

Complete Growth Medium	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing	Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 X 10⁵ viable cells/mL. Maintain cell density between 2 to 3 X 10⁵ and 1 to 2 X 10⁶ viable cells/mL. Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 3 days
Cryopreservation	Freeze Medium: Complete growth medium 95%; DMSO, 5% Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions	Temperature: 37°C

STR Profile	Amelogenin: X CSF1PO: 10,11 D13S317: 11,12 D16S539: 10,13 D5S818: 12,13 D7S820: 9,13 THO1: 6,7 TPOX: 8 vWA: 17,19
Isoenzymes	ADA, 1 ES-D, 1 G6PD, B GLO-I, 1 PEP-D, 1 PGD, C PGM1, 1 PGM3, 0

Name of Depositor	GE Foley
Deposited As	Homo sapiens
Year of Origin	November, 1964
References	Foley GE, et al. Continous culture of human lymphoblasts from peripheral blood of a child with acute leukemia. Cancer 18: 522-529, 1965. PubMed: 14278051 Sandstrom PA, Buttke TM. Autocrine production of extracellular catalase prevents apoptosis of the human CEM T-cell line in serum-free medium. Proc. Natl. Acad. Sci. USA 90: 4708-4712, 1993. PubMed: 8506323 Adams RA. Formal discussion: the role of transplantation in the experimental investigation of human leukemia and lymphoma. Cancer Res. 27: 2479-2482, 1967. PubMed: 4170381 Uzman BG, et al. Morphologic variations in human leukemic lymphoblasts (CCRF-CEM cells) after long-term culture and exposure to chemotherapeutic agents. A study with the electron microscope. Cancer 19: 1725-1742, 1966. PubMed: 5224274 Adams RA, et al. Leukemia: serial transplantation of human leukemic lymphoblasts in the newborn Syrian hamster. Cancer Res. 27: 772-783, 1967. PubMed: 4295047 Miranda L, et al. Isolation of the human PC6 gene encoding the putative host protease for HIV-1 gp160 processing in CD4+ T lymphocytes. Proc. Natl. Acad. Sci. USA 93: 7695-7700, 1996. PubMed: 8755538 CCRF-CEM is a T lymphoblastoid cell line derived by G.E. Foley, et al. using cells obtained in November, 1964 from peripheral blood buffy coat of a 4-year-old Caucasian female with acute lymphoblastic leukemia.

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis SDS
FAQ's	Agreement to transfer to a CRO Date Updated: 1/2/2013
References	Foley GE, et al. Continous culture of human lymphoblasts from peripheral blood of a child with acute leukemia. Cancer 18: 522-529, 1965. PubMed: 14278051 Sandstrom PA, Buttke TM. Autocrine production of extracellular catalase prevents apoptosis of the human CEM T-cell line in serum-free medium. Proc. Natl. Acad. Sci. USA 90: 4708-4712, 1993. PubMed: 8506323 Adams RA. Formal discussion: the role of transplantation in the experimental investigation of human leukemia and lymphoma. Cancer Res. 27: 2479-2482, 1967. PubMed: 4170381 Uzman BG, et al. Morphologic variations in human leukemic lymphoblasts (CCRF-CEM cells) after long-term culture and exposure to chemotherapeutic agents. A study with the electron microscope. Cancer 19: 1725-1742, 1966. PubMed: 5224274 Adams RA, et al. Leukemia: serial transplantation of human leukemic lymphoblasts in the newborn Syrian hamster. Cancer Res. 27: 772-783, 1967. PubMed: 4295047 Miranda L, et al. Isolation of the human PC6 gene encoding the putative host protease for HIV-1 gp160 processing in CD4+ T lymphocytes. Proc. Natl. Acad. Sci. USA 93: 7695-7700, 1996. PubMed: 8755538 CCRF-CEM is a T lymphoblastoid cell line derived by G.E. Foley, et al. using cells obtained in November, 1964 from peripheral blood buffy coat of a 4-year-old Caucasian female with acute lymphoblastic leukemia.