Certified Reference Materials (CRMs)

  • CCRF-CEM [CCRF CEM] (ATCC® CRM-CCL-119)

    ATCC® Number: CRM-CCL-119

    Organism: Homo sapiens, human
    Cell Type: T Lymphoblast
    Tissue:
    Peripheral Blood
    Disease Acute Lymphoblastic Leukemia

    For-Profit:   $714.00 Non-Profit:   $535.50

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    Tissue:
    Peripheral Blood
    Product Format: frozen
  • Human DNA for Whole-Genome Variant Assessment (Daughter of Utah/European Ancestry) (ATCC® NIST-8398)

    ATCC® Number: NIST-8398

    Storage: NIST-8398 is stored at −20°C for long-term storage, or in the dark at 4°C for short-term storage (if use is imminent). Please limit freeze-thaw cycles
    Applications: This Reference Material (RM) is intended for assessing performance of human genome sequencing, including whole genome sequencing, whole exome sequencing, and more targeted sequencing such as gene panels. Specifically, the material can be used to obtain estimates of true positives, false positives, true negatives, and false negatives for variant calls. This genomic DNA is to be analyzed as any other processed, extracted DNA. It can be used to assess sequencing library preparation, sequencing machines, and the bioinformatics steps of mapping, alignment, and variant calling. This RM is not intended to assess subsequent bioinformatics steps such as functional or clinical interpretation. 

    This RM is intended to provide a whole human genome sample and accompanying reference values to assess performance of variant calling from genome sequencing

    For-Profit:   $608.00 Non-Profit:   $608.00

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    Comments: This Reference Material (RM) is intended to provide a whole human genome sample and accompanying reference values to assess performance of variant calling from genome sequencing. This RM contains human genomic deoxyribonucleic acid (DNA) extracted from a large growth of the human lymphoblastoid cell line GM12878. A unit of RM 8398 consists of a single vial containing approximately 10 μg of genomic DNA, with the peak of the nominal length distribution longer than 48.5 kb, as referenced by Lambda DNA, and the DNA is in TE buffer (10 mM TRIS, pH 8.0, 1 mM EDTA, pH 8.0).

    See the NIST Certificate of Analysis for directions for use and quality control specifications. 

    Certificate of Analysis

    NIST SDS


  • RPMI 8226 (ATCC® CRM-CCL-155)

    ATCC® Number: CRM-CCL-155

    Organism: Homo sapiens, human
    Cell Type: B Lymphocyte (Kras Crm)
    Tissue:
    Peripheral Blood
    Disease Plasmacytoma; Myeloma

    For-Profit:   $714.00 Non-Profit:   $535.50

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    Tissue:
    Peripheral Blood
    Product Format: frozen
  • Mycoplasma salivarium (ATCC® qCRM-23064D)

    ATCC® Number: qCRM-23064D

    Strain Designations: Genomic DNA from Mycoplasma salivarium strain [H110, NCTC 10113, PG 20]
    Type Strain: yes
    Biosafety Level: 1

    For-Profit:   $490.00 Non-Profit:   $490.00

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    Biosafety Level: 1
    Product Format: frozen
    Specification range: 1x106 - 1x107 genome copies/µL
  • Mycoplasma pneumoniae Somerson et al. (ATCC® qCRM-15531D)

    ATCC® Number: qCRM-15531D

    Strain Designations: Genomic DNA from Mycoplasma pneumoniae FH strain of Eaton Agent [NCTC 10119]
    Type Strain: yes
    Biosafety Level: 1

    For-Profit:   $490.00 Non-Profit:   $490.00

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    Biosafety Level: 1
    Product Format: frozen
    Specification range: 1x106 - 1x107 genome copies/µL
  • Mycoplasma orale (ATCC® qCRM-23714D)

    ATCC® Number: qCRM-23714D

    Strain Designations: Genomic DNA from Mycoplasma orale strain CH 19299 [NCTC 10112]
    Type Strain: yes
    Biosafety Level: 1

    For-Profit:   $490.00 Non-Profit:   $490.00

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    Biosafety Level: 1
    Product Format: frozen
    Specification range: 1x106 - 1x107 genome copies/µL
  • High molecular weight genomic DNA isolated from A-549 Lung Carcinoma cell line. (ATCC® CRM-CCL-185D)

    ATCC® Number: CRM-CCL-185D

    Quantity: 5 μg
    Storage: -80°C or colder
    Applications: For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation. ISO Guide 34:2009.

    For-Profit:   $408.00 Non-Profit:   $408.00

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    Product Format: 5 μg
    Comments:

    Store at - 80°C or colder in a manual defrost freezer.  Avoid freeze-thawing.  Alternatively, product can be stored at -20°C or colder, but stability at this temperature has not been tested by the ATCC and is not guaranteed.

    Certified Reference Material produced under an ISO Guide 34:2009 accredited process.

    This item has been tested for KRAS mutation: Homozygous DNA Change: c.34G>A (correlates to protein sequence p.G12S).

  • Candida albicans (Robin) Berkhout (ATCC® CRM-10231)

    ATCC® Number: CRM-10231

    Deposited As Candida albicans (Robin) Berkhout, anamorph
    Alternate State: Monilia pinoyi (Castellani) Castellani et Chalmers; Endomyces albicans (Robin) Vuillemin; Candida claussenii Lodder et Kreger-van Rij, anamorph; Candida stellatoidea (Jones et Martin) Langeron et Guerra, anamorph
    Designation: 3147 [CBS 6431, CCY 29-3-106, CIP 48.72, DSM 1386, IFO 1594, NCPF 3179, NCYC 1363, NIH 3147, VTT C-85161]

    For-Profit:   $420.00 Non-Profit:   $315.00

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    Comments: Certificates of Analysis are available electronically at www.atcc.org, or by hardcopy upon request.

    Certified Reference Material produced under an ISO Guide 34:2009 accredited process. For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation.

    For sterility testing, not more than five passages from the ATCC culture should be used.

    Type Strain: no
    Biological Safety Level: 1
  • SRM Genomic DNA Standards for HER2 Measurements (ATCC® NIST-2373)

    ATCC® Number: NIST-2373

    Quantity: 100 µL of DNA solution per vial
    Applications: Used to assign ratios of the human epidermal growth factor 2 gene (HER2; the official gene symbol ERBB2) to unamplified reference genes. Measurements of the amplification (increased copies) of the HER2 gene in breast cancer samples are used as a biomarker for determining the classification for breast cancers

    For-Profit:   $1,473.00 Non-Profit:   $1,473.00

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    Product Format: 100 µL of DNA solution per vial
    Comments: SRM NIST-2373 consists of genomic DNA extracted from five breast cancer cell lines with different amounts of amplification of the HER2 gene. The five purified genomic DNAs were solubilized in a buffer consisting of 10 mmol/L tris(hydroxymethyl)aminomethane and 0.1 mmol/L ethylenediaminetetraacetic acid disodium salt (EDTA) pH 8.0 (TE–4). The five components are genomic DNA materials derived from human cell lines SK-BR-3, MDA-MB-231, MDA-MB-361, MDA-MB-453, and BT-474, labeled A, B, C, D, and E, respectively. A unit of SRM 2373 consists of five vials, one of each component, containing approximately 100 μL of DNA solution. Each of these vials is labeled and is sealed with a color-coded screw cap.

    See the NIST Certificate of Analysis for directions for use and quality control specifications. 

    Certificate of Analysis

    NIST SDS

  • Mycoplasma hominis (ATCC® qCRM-27545D)

    ATCC® Number: qCRM-27545D

    Strain Designations: Genomic DNA from Mycoplasma hominis strain [LBD-4]
    Type Strain: no
    Biosafety Level: 1

    For-Profit:   $490.00 Non-Profit:   $490.00

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    Biosafety Level: 1
    Product Format: frozen
    Specification range: 1x106 - 1x107 genome copies/µL