Certified Reference Materials (CRMs)

  • Ca Ski (ATCC® CRM-CRL-1550)

    ATCC® Number: CRM-CRL-1550

    Organism: Homo sapiens, human
    Cell Type: Epithelial Cell (Crm)
    Tissue: Cervix; Derived Fom Metastatic Site: Small Intestine
    Disease Epidermoid Carcinoma

    For-Profit:   $611.00 Non-Profit:   $519.35

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    Tissue: Cervix
    Product Format: frozen
  • SRM PCR-Based DNA Profiling Standard (ATCC® NIST-2391c)

    ATCC® Number: NIST-2391c

    Storage: Store in the dark between 2°C to 8°C. DO NOT FREEZE.
    Applications: For use in the standardization of forensic and paternity quality assurance procedures for polymerase chain reaction (PCR)-based genetic testing, for instructional law enforcement or non-clinical research purposes, and for quality assurance when assigning values to in-house control materials. It is not intended for any human or animal clinical diagnostic use.

    For-Profit:   $1,119.00 Non-Profit:   $1,119.00

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    Comments: SRM NIST-2391c is composed of well-characterized human deoxyribonucleic acid (DNA) in two forms: genomic DNA (Components A through D) and DNA to be extracted from cells that have been spotted onto 903 paper (Component E) and FTA paper (Component F).

     

    Component Description Amount Concentration(a)
    A Anonymous single-source female genomic DNA in TE-4 buffer 50 μL 1.1 – 2.1 ng/μL
    B Anonymous single-source male genomic DNA in TE-4 buffer 50 μL 1.1 – 2.1 ng/μL
    C Anonymous single-source male genomic DNA in TE-4 buffer 50 μL 1.1 – 2.1 ng/μL
    D Mixed-source (Components A and C) genomic DNA in TE-4 buffer 50 μL 1.1 – 2.1 ng/μL
    E Anonymous single-source female cells spotted on 903 paper Two 6 mm punches 7.5 × 104 cells per punch
    F Anonymous single-source male cells spotted on FTA paper Two 6 mm punches 7.5 × 104 cells per punch

     

    See the NIST Certificate of Analysis for directions for use and quality control specifications. 

    Certificate of Analysis

    NIST SDS

     

  • SRM Genomic DNA Standards for HER2 Measurements (ATCC® NIST-2373)

    ATCC® Number: NIST-2373

    Quantity: 100 µL of DNA solution per vial
    Applications: Used to assign ratios of the human epidermal growth factor 2 gene (HER2; the official gene symbol ERBB2) to unamplified reference genes. Measurements of the amplification (increased copies) of the HER2 gene in breast cancer samples are used as a biomarker for determining the classification for breast cancers

    For-Profit:   $1,473.00 Non-Profit:   $1,473.00

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    Product Format: 100 µL of DNA solution per vial
    Comments: SRM NIST-2373 consists of genomic DNA extracted from five breast cancer cell lines with different amounts of amplification of the HER2 gene. The five purified genomic DNAs were solubilized in a buffer consisting of 10 mmol/L tris(hydroxymethyl)aminomethane and 0.1 mmol/L ethylenediaminetetraacetic acid disodium salt (EDTA) pH 8.0 (TE–4). The five components are genomic DNA materials derived from human cell lines SK-BR-3, MDA-MB-231, MDA-MB-361, MDA-MB-453, and BT-474, labeled A, B, C, D, and E, respectively. A unit of SRM 2373 consists of five vials, one of each component, containing approximately 100 μL of DNA solution. Each of these vials is labeled and is sealed with a color-coded screw cap.

    See the NIST Certificate of Analysis for directions for use and quality control specifications. 

    Certificate of Analysis

    NIST SDS

  • Mycoplasma arginini (ATCC® qCRM-23838D)

    ATCC® Number: qCRM-23838D

    Strain Designations: Genomic DNA from Mycoplasma arginini strain G230 [NCTC 10129]
    Type Strain: yes
    Biosafety Level: 1

    For-Profit:   $490.00 Non-Profit:   $490.00

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    Biosafety Level: 1
    Product Format: frozen
    Specification range: 1x106 - 1x107 genome copies/µL
  • High molecular weight genomic DNA isolated from CCRF-CEM; Leukemia; Human cell line. (ATCC® CRM-CCL-119D)

    ATCC® Number: CRM-CCL-119D

    Quantity: 5 μg
    Storage: - 80°C or colder
    Applications:

    For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation. ISO Guide 34:2009.

    For-Profit:   $408.00 Non-Profit:   $408.00

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    Product Format: frozen 5 μg
    Comments:

    Certificates of Analysis are available electronically at www.atcc.org, or by hardcopy upon request.

    Certified Reference Material produced under an ISO Guide 34:2009 accredited process.

    This item has been tested for KRAS mutation: Heterozygous DNA Change c.35G>A (correlates to protein sequence p.G12D) 

  • Mycoplasma pneumoniae Somerson et al. (ATCC® qCRM-15531D)

    ATCC® Number: qCRM-15531D

    Strain Designations: Genomic DNA from Mycoplasma pneumoniae FH strain of Eaton Agent [NCTC 10119]
    Type Strain: yes
    Biosafety Level: 1

    For-Profit:   $490.00 Non-Profit:   $490.00

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    Biosafety Level: 1
    Product Format: frozen
    Specification range: 1x106 - 1x107 genome copies/µL
  • High molecular weight genomic DNA isolated from NCI-H441 Lung Adenocarcinoma; Human cell line (ATCC® CRM-HTB-174D)

    ATCC® Number: CRM-HTB-174D

    Quantity: 5 μg
    Storage: -80°C or colder
    Applications:

    For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation. ISO Guide 34:2009.

    For-Profit:   $408.00 Non-Profit:   $408.00

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    Product Format: frozen 5 μg
    Comments:

    Store at - 80°C or colder in a manual defrost freezer.  Avoid freeze-thawing.  Alternatively, product can be stored at -20°C or colder, but stability at this temperature has not been tested by the ATCC and is not guaranteed.

    Certified Reference Material produced under an ISO Guide 34:2009 accredited process.

    This item has been tested for KRAS mutation: Heterozygous DNA Change c.35G>T (correlates to protein sequence p.G12V).

  • High molecular weight genomic DNA isolated from MDA-MB-231; Breast Adenocarcinoma; Human cell line (ATCC® CRM-HTB-26D)

    ATCC® Number: CRM-HTB-26D

    Quantity: 5 μg
    Storage: -80°C or colder
    Applications:

    For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation. ISO Guide 34:2009.

    For-Profit:   $408.00 Non-Profit:   $408.00

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    Product Format: frozen 5 μg
    Comments:

    Store at - 80°C or colder in a manual defrost freezer.  Avoid freeze-thawing.  Alternatively, product can be stored at -20°C or colder, but stability at this temperature has not been tested by the ATCC and is not guaranteed.

    Certified Reference Material produced under an ISO Guide 34:2009 accredited process.

    This item has been tested for KRAS mutation: Heterozygous DNA Change c.38G>A (correlates to protein sequence p.G13D). 

  • Pseudomonas aeruginosa (Schroeter) Migula (ATCC® CRM-27853)

    ATCC® Number: CRM-27853

    Strain Designations: Boston 41501
    Type Strain: no
    Biosafety Level: 2

    For-Profit:   $420.00 Non-Profit:   $357.00

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    Biosafety Level: 2
    Product Format: freeze-dried
  • Candida albicans (Robin) Berkhout (ATCC® CRM-10231)

    ATCC® Number: CRM-10231

    Deposited As Candida albicans (Robin) Berkhout, anamorph
    Alternate State: Monilia pinoyi (Castellani) Castellani et Chalmers; Endomyces albicans (Robin) Vuillemin; Candida claussenii Lodder et Kreger-van Rij, anamorph; Candida stellatoidea (Jones et Martin) Langeron et Guerra, anamorph
    Designation: 3147 [CBS 6431, CCY 29-3-106, CIP 48.72, DSM 1386, IFO 1594, NCPF 3179, NCYC 1363, NIH 3147, VTT C-85161]
    Product Format: freeze-dried

    For-Profit:   $420.00 Non-Profit:   $357.00

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    Comments: Certificates of Analysis are available electronically at www.atcc.org, or by hardcopy upon request.

    Certified Reference Material produced under an ISO Guide 34:2009 accredited process. For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation.

    For sterility testing, not more than five passages from the ATCC culture should be used.

    Type Strain: no
    Biological Safety Level: 1