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pBR313 (ATCC® 37018™)

Applications: Food testing  /  Depositors: H Boyer

Designations pBR313
Permits and Restrictions

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Depositors H Boyer
Biosafety Level 1
Vector Information
Size (kb): 9.600
Vector: pBR313 (plasmid)
Marker(s):ampR,tetR
Construct size (kb): 9.600
Features: marker(s): ampR, tetR
replicon: pMB1
Applications
Food testing
Comments
General purpose plasmid vector.
Negative control for dectection of heat-labile enterotoxin.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Bolivar F, et al. Construction and characterization of new cloning vehicles. I. Ampicillin-resistant derivatives of the plasmid pMB9. Gene 2: 75-93, 1977. PubMed: 344136

AOAC International Detection of Escherichia coli producing heat-labile enterotoxin. DNA colony hybridization method. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 984.34.

Shipped frozen