MS2 bacteriophage (ATCC® 15597-B1)

Strain Designations: MS2  /  Type Strain: no  /  Biosafety Level: 1

Deposited As MS2
Strain Designations MS2
Application
Water testing
Control Culture Ref USEPA Manuual of Method for Virology, Chapeter 16. Washington, DC:Environmental Protection Agency;EPA EPA 600/4-84/013 (N16), 2001
coliphages in water Ref 9224 C: Male-specific coliphage assay using Escherichia coli Famp. Washington, DC:American Public Health Association;Standard Methods for the Examination of Water and Wastewater, 2005 APHA9224 - Detection of Coliphages;.
control strain RefEnvironmental Protection Agency Male-specific (F+) and Somatic Coliphage in Water by Two-Step Enrichment Procedure. Washington, DC:Environmental Protection Agency;EPA EPA Method 1601, 2001 RefEnvironmental Protection Agency Male-specific (F+) and Somatic Coliphage in Water by Single Agar Layer (SAL) Procedure. Washington, DC:Environmental Protection Agency;EPA EPA Method 1602, 2001
detection of bacteriophages Ref Water quality --- Detection and enumeration of bacteriophages --- Part 1: Enumeration of F-specific RNA bacteriophages [BS EN ISO 10705-1:2001]. London, UK:British Standards Institution;British Standard BS 6068-4.11:1996.
testing Ref Inactivation of MS2 virus in Drinking Water. Washington, DC:Environmental Protection Agency;EPA EPA NSF 02/03/EPADWCTR. Ref Protocal for Equipment Verification Testing for Physical Removal of Microbiological and Particulate Contaminants. Washington, DC:Environmental Protection Agency;EPA EPA 05/9205/EPADWCTR.
Type Strain no
Biosafety Level 1
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® yes
Type Strain no
Colony Morphology male-specific (F+) coliphage
Medium ATCC® Medium 271: Escherichia medium
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Phenotype male-specific (F+) coliphage
Colony Morphology male-specific (F+) coliphage
Name of Depositor JH Strauss
Chain of Custody
ATCC <-- JH Strauss <-- A Clark
References

Strauss H Jr., Sinsheimer RL. Purification and properties of bacteriophage MS2 and of its ribonucleic acid. J. Mol. Biol. 7: 43-54, 1963. PubMed: 13978804

Environmental Protection Agency Male-specific (F+) and Somatic Coliphage in Water by Two-Step Enrichment Procedure. Washington, DC:Environmental Protection Agency;EPA EPA Method 1601, 2001

Environmental Protection Agency Male-specific (F+) and Somatic Coliphage in Water by Single Agar Layer (SAL) Procedure. Washington, DC:Environmental Protection Agency;EPA EPA Method 1602, 2001

Inactivation of MS2 virus in Drinking Water. Washington, DC:Environmental Protection Agency;EPA EPA NSF 02/03/EPADWCTR.

Protocal for Equipment Verification Testing for Physical Removal of Microbiological and Particulate Contaminants. Washington, DC:Environmental Protection Agency;EPA EPA 05/9205/EPADWCTR.

USEPA Manuual of Method for Virology, Chapeter 16. Washington, DC:Environmental Protection Agency;EPA EPA 600/4-84/013 (N16), 2001

Water quality--Detection and enumeration of bacteriophages--Part 1: Enumeration of F-specific RNA bacteriophages. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 10705-1:1995.

9224 C: Male-specific coliphage assay using Escherichia coli Famp. Washington, DC:American Public Health Association;Standard Methods for the Examination of Water and Wastewater, 2005 APHA9224 - Detection of Coliphages;.

Water quality --- Detection and enumeration of bacteriophages --- Part 1: Enumeration of F-specific RNA bacteriophages [BS EN ISO 10705-1:2001]. London, UK:British Standards Institution;British Standard BS 6068-4.11:1996.

Fast Phage Test. Charm.