Human herpesvirus 5 (ATCC® VR-538)

Classification: Herpesviridae, Cytomegalovirus  /  Product Format: frozen 1 mL per vial

Classification Herpesviridae, Cytomegalovirus
Deposited As Cytomegalovirus
Agent Human herpesvirus 5
Strain AD-169
Common Name Human cytomegalovirus
Applications
Testing virucides
Sexually Transmitted Disease Research
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions Liquid nitrogen vapor phase
Genome Sequenced Strain

Yes

Images VR-538
Comments
Preserved in virus growth medium with 7% DMSO.
GenBank: X17403.
Effect on Host CPE, cell enlarging, rounding, sloughing
Recommended Host
MRC-5 (ATCC® CCL-171™)
Growth Conditions Temperature: 36°C
Recommendations for Infection: This product is produced by co-cultivation of virus with fresh host cells. Prepare a bulk cell suspension the day of inoculation. Seed culture vessels at 1.5 - 2.0 x 104 cells per cm2. Calculate the volume of virus needed to achieve an optimal MOI (e.g. 0.1 to 1) and then dilute virus in virus growth medium to prepare the virus inoculum. Add virus inoculum to culture vessels. Incubate for 24 hours at 36°C in a humidified 5% CO2 atmosphere. Aspirate virus growth medium to remove any traces of DMSO and then add fresh virus growth medium to cultures. Continue incubation.
Incubation: 4 to 11 days
Effect on Host CPE, cell enlarging, rounding, sloughing
Name of Depositor WA Chappell
Special Collection NCRR Contract
Source Adenoid tissue from 7-year-old female
References

ASTM International Standard Test Method for Efficacy of Antimicrobial Agents Against Viruses in Suspension. West Conshohocken, PA (Reapproved 2002).

ASTM International Standard Test Method for Efficacy of Virucidal Agents Intended for Inanimate Environmental Surfaces. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1053-97 (Reapproved 2002).

Huang L, et al. The variable e' endds of a human cytomegalovirus oriLyt transcript (SRT) overlap an essential, conserved replicator element. J. Virol. 70: 5272-5281, 1996. PubMed: 8764037

Iskenderian AC, et al. Four of eleven loci required for transient complementation of human cytomegalovirus DNA replication cooperate to activate expression of replication genes. J. Virol. 70: 383-392, 1996. PubMed: 8523552

Noraz N, et al. Human cytomegalovirus-associated immunosuppression is mediated through interferon-alpha. Blood 89: 2443-2452, 1997. PubMed: 9116288

Prichard MN, et al. Human cytomegalovirus uracil DNA glycosylase is required for the normal temporal regulation of both DNA synthesis and viral replication. J. Virol. 70: 3018-3025, 1996. PubMed: 8627778

Rowe WP, et al. Cytopathogenic agent resembling human salivary gland virus recovered from tissue cultures of human adenoids. Proc. Soc. Exp. Biol. Med. 92: 418-424, 1956. PubMed: 13350367