Human rotavirus (ATCC® VR-2018)

Classification: Reoviridae, Rotavirus, Rotavirus A  /  Product Format: frozen 1 mL per vial

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Classification Reoviridae, Rotavirus, Rotavirus A
Agent Human rotavirus
Strain Wa (TC adapted)
Applications
Efficacy testing
Testing
Testing virucides
Viruscide testing
Enteric Research
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments VP7 serotype 1 (G1).
Effect on Host
CPE, refractile rounding and sloughing.
Recommended Host
MA-104 Clone 1 (ATCC® CRL-2378.1™)
Growth Conditions Temperature: 37°C
Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere while continuously rocking. End adsorption by adding virus growth medium.
Incubation: 1-7 days
Effect on Host
CPE, refractile rounding and sloughing.
Name of Depositor RG Wyatt, National Institute of Allergy and Infectious Disease
Source Diarrhea stool from patient positive for rotavirus
References

ASTM International Standard Test Method for Efficacy of Antimicrobial Agents Against Viruses in Suspension. West Conshohocken, PA (Reapproved 2002).

ASTM International Standard Test Method for Efficacy of Virucidal Agents Intended for Inanimate Environmental Surfaces. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1053-97 (Reapproved 2002).

Standard Quantitative Disk Carrier Test Method for Determining the Bactericidal, Virucidal, Fungicidal, Mycobactericidal and Sporicidal Activities of Liquid Chemical Germicides. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 2197-02.

Standard Test Method for Determining the Virus-Eliminating Effectiveness of Liquid Hygienic Handwash and Handrub Agents Using the Fingerpads of Adult Volunteers. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1838-02.

Standard Test Method for Evaluation of Handwashing Formulation for Virus-Eliminating Activity using the Entire Hand. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 2011-99.

Wyatt RG, et al. Cultivatable human rotavirus type 2. US Patent 4,341,870 dated Jul 27 1982

Water supply -- Domestic type water treatment appliances -- Performance requirements. Sydney, NSW, Australia:Standards Australia;Standards Australia AS/NZS 4348:1995.

Wyatt RG, James WD. Methods of gastroenteritis virus culture in vivo and in vitro In: Tyrrell AAJ, Kapikian ZA Viral infections of the gastrointestinal tract Marcel Dekker, New York, pp. 13-35, 1982

Wyatt RG, et al. Human rotavirus type 2: cultivation in vitro. Science 207: 189-191, 1980. PubMed: 6243190

Cross References

Nucleotide (GenBank) : AF200224 Human rotavirus strain Wa clone 1 nonstructural protein NSP4 mRNA, complete cds.

Nucleotide (GenBank) : AF200225 Human rotavirus strain Wa clone 2 nonstructural protein NSP4 mRNA, complete cds.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation