Canine parvovirus (ATCC® VR-2017)

Classification: Parvoviridae, Parvovirus, Feline panleukopenia virus  /  Product Format: frozen

Permits and Restrictions

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Classification Parvoviridae, Parvovirus, Feline panleukopenia virus
Agent Canine parvovirus
Strain Cornell-780916-80
Efficacy testing
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions  -70°C or colder 
Reduced virulence variant obtained by passage at 33°C.
For best titer and CPE, infect actively growing cells. Virus agglutinates pig and rhesus monkey erythrocytes.
Effect on Host
CPE; refractile cell rounding and sloughing
Recommended Host
A-72 (ATCC® CRL-1542™)
Growth Conditions Temperature: 37°C
Incubation: Incubate infected A-72 cell culture for 3-5 days at 37°C in 100% atmospheric air, until CPE is well advanced through 90% of the culture.
Recommendations for Infection:  Plant cells 6 hours in advance and infect when cultures are 40-50% confluent.  Remove medium and inoculate with a small volume (e.g. 1 mL per 25 cm2 ) of virus diluted with virus growth medium to provide a MOI of about 1.0. Adsorb 1 hour at 37°C in 100% atmospheric air in nonvented flasks.  End adsorption by adding viral growth media.
Effect on Host
CPE; refractile cell rounding and sloughing
Name of Depositor Cornell Res. Fndn., Inc.
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
Source Intestinal contents of beagle pups with severe enteritis

Carmichael LE, et al. Hemagglutination by canine parvovirus: serologic studies and diagnostic applications. Am. J. Vet. Res. 40: 784-791, 1980. PubMed: 6250432

Standard Quantitative Disk Carrier Test Method for Determining the Bactericidal, Virucidal, Fungicidal, Mycobactericidal and Sporicidal Activities of Liquid Chemical Germicides. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 2197-02.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation