Hybrid Moloney/Amphotropic murine leukemia virus (Mo/A-MuLv) (ATCC® VR-1450)

Classification: Retrovirus, Mammalian Type C Retrovirus, Mammalian Group, Amphotropic  /  Product Format: frozen

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Classification Retrovirus, Mammalian Type C Retrovirus, Mammalian Group, Amphotropic
Agent Hybrid Moloney/Amphotropic murine leukemia virus (Mo/A-MuLv)
Strain 4070A envelope strain
Applications
Analytical methodologies
Pharmaceutical and Personal Care
Biosafety Level 2
Isolation
Virus was harvested from tk(-) NIH3T3 cells transfected with molecular clone of MLV (VR-1448)
Product Format frozen
Storage Conditions -70°C or colder
Comments
This virus produces plaques on PG-4 (feline S+L-) cells (ATCC CRL-2032), and foci of rounded cells on MiCL1 (Mink S+L-) cells (ATCC CCL-64.1). This holding is prepared by growing ATCC VR-1448 and harvesting the supernate.
At the time of deposit, cell-free preparations of the Mo/A-MuLV strain were considered as suitable reference standards in assays to detect replication competent murine retrovirus--select lots have had titer confirmed by workers outside the ATCC.
amphotropic
Effect on Host
No, in vitro effects: Cytopathic effects in NIH3T3 cells
No CPE in NIH3T3 cells
Recommended Host
NIH/3T3 (tk-) (ATCC CRL-1658)

Recommended Host: tk(-) NIH3T3 (ATCC® CRL-1658™)

many mammalian cell lines (mouse, rat, human)
Growth Conditions
Duration: 5-7 days at 37.0°C in NIH/3T3 (tk-) (ATCC CRL-1658)
Cryopreservation
Storage temperature: -70C
Effect on Host
No, in vitro effects: Cytopathic effects in NIH3T3 cells
No CPE in NIH3T3 cells
Name of Depositor CA Wilson
Special Collection NCRR Contract
Source
Virus was harvested from tk(-) NIH3T3 cells transfected with molecular clone of MLV (VR-1448)
References

Miller AD, et al. Generation of helper-free amphotropic retroviurses that transduce a dominant-acting, methotrexate-resistant dihydrofolate reductase gene. Mol. Cell. Biol. 5: 431-437, 1985. PubMed: 2985952

General Chapters: <1046> Cell and Gene Therapy Products - Analytical Methodologies. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011

Cross References

Nucleotide (GenBank) : AF010170 Plasmid pAMS with hybrid amphotropic/Moloney murine leukemia virus, complete sequence.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation