MDBK (NBL-1) (ATCC® CCL-22)

Organism: Bos taurus, cow  /  Tissue: kidney  / 

Organism Bos taurus, cow
Tissue kidney
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Age adult
Gender male
Applications
This cell line is suitable as a transfection host
Storage Conditions liquid nitrogen vapor phase
Karyotype Chromosome Frequency Distribution 50 Cells: 2n = 60. The stemline chromosome number is hypodiploid with 2S component occurring at 5%., There were a total of 11-14 marker chromosomes (4-5 metacentric, 3-4 submetacentric and 4-5 acro-telocentric) common to most hypodiploid metaphases. The X was monosomic. Neither HSR chromosomes nor DM's were seen.
Derivation
The MDBK cell line was derived from a kidney of an apparently normal adult steer, February 18, 1957, by S.H. Madin and N.B. Darby. The cells were originally obtained in July, 1967 at passage 96 from the ATCC as a frozen ampule (F-145).­
Clinical Data
male
Genes Expressed
keratin
Virus Susceptibility Vesicular stomatitis, Orsay (Indiana)
Infectious bovine rhinotracheitis
Bovine parvovirus
Bovine adenovirus 2
Bovine adenovirus 3
Bovine viral diarrhea virus 1 , Bovine viral diarrhea virus 1
Parainfluenza 3
Virus Resistance
poliovirus 2
Comments
It was ascertained in 1973 that the MDBK line was infected with bovine viral diarrhea virus (BVDV).
In 1982 R.A. Van Deusen of the NVSL, USDA, Ames, Iowa informed the ATCC that they had found an MDBK free of BVDV infection.

The line has been shown to be free of bovine viral diarrhea virus (BVDV).

The cells are positive for keratin by immunofluorescence. 

Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Subculturing

Rinse the cell sheet twice with fresh 0.25% trypsin, 0.03% EDTA solution, and remove the trypsin solution. Allow the flask to stand at room temperature for 10 to 15 minutes, add fresh medium, aspirate and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: Twice per week

Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Name of Depositor S Madin, NB Darby
Passage History

The cell line was submitted to the American Type Culture Collection by R.A. Van Deusen in passage 110 in August, 1982.

The cells were originally obtained in July, 1967 at passage 96 from the ATCC as a frozen ampule (F-145). Extensive testing (35 passages) by the NVSL found no evidence of any viral contamination in this particular progeny of MDBK.

Year of Origin 1957
References

Madin SH, Darby NB Jr.. Established kidney cell lines of normal adult bovine and ovine origin. Proc. Soc. Exp. Biol. Med. 98: 574-576, 1958. PubMed: 13567776

Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438

Loffler S, et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321

Russell DW, Miller AD. Foamy virus vectors. J. Virol. 70: 217-222, 1996. PubMed: 8523528

USEPA Manual of Methods for Virology - EPA publication. Washington, DC:Environmental Protection Agency;EPA EPA 600/4-84/013 (R9), 2001

Cross References

Nucleotide (GenBank) : AF025996 Bos taurus supervillin mRNA, complete cds.

Nucleotide (GenBank) : U64997 Bos taurus ribonuclease K6 gene, partial cds.

Nucleotide (GenBank) : NC_002531 Alcelaphine herpesvirus 1, complete genome.

Nucleotide (GenBank) : AJ238405 Bos taurus mRNA for elongation factor 1 alpha.

Nucleotide (GenBank) : J05217 Bovine beta-1->4-galactosyltransferase mRNA, 5' end.

Nucleotide (GenBank) : AF005370 Alcelaphine herpesvirus 1 L-DNA, complete sequence.

Basic Documentation
References

Madin SH, Darby NB Jr.. Established kidney cell lines of normal adult bovine and ovine origin. Proc. Soc. Exp. Biol. Med. 98: 574-576, 1958. PubMed: 13567776

Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438

Loffler S, et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321

Russell DW, Miller AD. Foamy virus vectors. J. Virol. 70: 217-222, 1996. PubMed: 8523528

USEPA Manual of Methods for Virology - EPA publication. Washington, DC:Environmental Protection Agency;EPA EPA 600/4-84/013 (R9), 2001