Klebsiella pneumoniae subsp. pneumoniae (Schroeter) Trevisan (ATCC® 700603)

Strain Designations: K6 [CCUG 45421, LMG 20218, MCV37]  /  Type Strain: no  /  Biosafety Level: 2

Deposited As Klebsiella pneumoniae (Schroeter) Trevisan
Strain Designations K6 [CCUG 45421, LMG 20218, MCV37]
Application
Produces beta-lactamase SHV-18
Quality control strain
Resistant to ampicillin
Resistant to aztreonam
Resistant to cefoxitin
Resistant to cefpodoxime
Resistant to ceftazidime
Resistant to chloramphenicol
Resistant to piperacillin
Resistant to tetracycline
Respiratory research
Sensitive to amoxicillin-clavulanate
Sensitive to cefepime
Intermediate resistance for cefotaxime
Sensitive to ciprofloxacin
Sensitive to imipenem
Sensitive to piperacillin-tazobactam
Intermediate resistance for Tobramycin
Sensitive to trimethoprim-sulfamethoxazole
Susceptibility disc testing
Susceptibility testing
Control for extended-spectrum beta-lactamase production
Isolation
Urine from hospitalized patient, Richmond, VA, 1994
Type Strain no
Biosafety Level 2
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Type Strain no
Antibiotic Resistance
Resistant to cefoxitin
Resistant to cefpodoxime
Resistant to ceftazidime
Resistant to chloramphenicol
Resistant to piperacillin
Resistant to ceftriaxone
Sensitive to trimethoprim-sulfamethoxazole
Sensitive to imipenem
Resistant to aztreonam
Resistant to ampicillin
Resistant to tetracycline
Sensitive to amoxicillin-clavulanate
Sensitive to cefepime
Sensitive to cefotaxime
Sensitive to ciprofloxacin
Sensitive to piperacillin-tazobactam
Intermediate resistance to gentamicin
Comments MIC (µg/mL):
Ampicillin >64
Amoxicillin-clavulanate 8
Aztreonam 64
Aztreonam-clavulanate <=0.25
Cefotaxime 8
Cefotaxime-clavulanate <=0.25
Cefpodoxime 16
Cefpodoxime-clavulanate 1
Ceftazidime 32
Ceftazidime-clavulanate 1
Ceftriaxone 16
Ceftriaxone-clavulanate <=0.25
Cefepime 1
Cefoxitin 32
Chloramphenicol >32
Ciprofloxacin 0.5
Gentamicin 8
Imipenem <=1
Piperacillin >128
Piperacillin-tazobactam 16
Tetracycline 16
Tobramycin 4
Trimethoprim-sulfamethoxazole 2
Medium ATCC® Medium 3: Nutrient agar or nutrient broth
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Name of Depositor JM Swenson
Special Collection NCRR Contract
Chain of Custody
ATCC <-- JM Swenson <-- MJ Ferraro <-- G Jacoby <-- H Dalton
Isolation
Urine from hospitalized patient, Richmond, VA, 1994
References

Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard - 9th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M2-A9.

Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard - 7th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M7-A8.

Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Approved Standard - 2nd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M31-A3.

Rasheed JK, et al. Characterization of the extended-spectrum beta-lactamase reference strain, Klebsiella pneumoniae K6 (ATCC 700603), which produces the novel enzyme SHV-18. Antimicrob. Agents Chemother. 44: 2382-2388, 2000. PubMed: 10952583

Performance Standards for Antimicrobial Susceptibility Testing; Sixteenth Informational Supplement. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M100-S20, 2007

BD Phoenix Gram Negative AST NMIC/ID QC Set. BD Becton Dickinson; Phoenix

MicroScan Dried Gram Negative Panel QC Set. Dade/Siemens.

Sensititre™ MIC Susceptibility. Thermo Scientific™ Sensititre™ Systems, Trek Diagnostic Systems.

Jana M Swenson, personal communication

VITEK 2 AST-GN QC Set. bioMerieux.

MicroScan ESBL plus QC Set. Dade/Siemens.

MicroScan Synergies plus Gram Negative QC Set. Dade/Siemens.

ETEST ESBL STRIP. bioMerieux.

Cross References

Nucleotide (GenBank) : AX111170 Sequence 1903 from Patent WO0123604.

Nucleotide (GenBank) : AF132290 Klebsiella pneumoniae plasmid-encoded extended-spectrum beta-lactamase (bla SHV-18) gene, complete cds.