pBR313 (ATCC® 37018)

Applications: Food testing  /  Depositors: H Boyer

Permits and Restrictions

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Designations pBR313
Depositors H Boyer
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 9.600
Vector: pBR313 (plasmid)
Construct size (kb): 9.600
Features: marker(s): ampR, tetR
replicon: pMB1
Food testing
General purpose plasmid vector.
Negative control for dectection of heat-labile enterotoxin.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Bolivar F, et al. Construction and characterization of new cloning vehicles. I. Ampicillin-resistant derivatives of the plasmid pMB9. Gene 2: 75-93, 1977. PubMed: 344136

AOAC International Detection of Escherichia coli producing heat-labile enterotoxin. DNA colony hybridization method. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 984.34.

Shipped frozen